Marine Biological Journal https://marine-biology.ru/mbj <p>Морской биологический журнал Marine Biological Journal.</p> <div><em><strong>Launched in February 2016.</strong></em></div> <div><em><strong>Certificates of registration:</strong></em></div> <div>print version: <a href="https://marine-biology.ru/public/journals/1/doc/registry_print.pdf" target="_blank" rel="noopener">ПИ № ФС 77 - 76872 of 24.09.2019</a>.</div> <div> <div><em><strong>Founder:</strong></em></div> <div>A. O. Kovalevsky Institute of Biology of the Southern Seas of RAS.</div> </div> <div><em><strong>Publishers</strong></em>:</div> <div><a href="http://ibss-ras.ru/" target="_blank" rel="noopener">A. O. Kovalevsky Institute of Biology of the Southern Seas of RAS</a>,</div> <div><a href="https://www.zin.ru/" target="_blank" rel="noopener">Zoological Institute of RAS</a>.</div> <div>ISSN 2499-9768 print.</div> <div><em><strong>Languages: </strong></em>Russian, English.</div> <div><em><strong>Periodicity:</strong></em> four issues a year.</div> <div> </div> <div><strong>Authors do not need to pay an article-processing charge.</strong></div> <div>The payment of royalties is not provided.</div> <div> </div> <div>Author recieves one copy of printed version of the journal as well as .pdf file.</div> <div> </div> <div> <div class="siteorigin-widget-tinymce textwidget"> <p>Marine Biological Journal is an open access, peer reviewed (double-blind) journal. The journal publishes original articles as well as reviews and brief reports and notes focused on new data of theoretical and experimental research in the fields of marine biology, diversity of marine organisms and their populations and communities, patterns of distribution of animals and plants in the World Ocean, the results of a comprehensive studies of marine and oceanic ecosystems, anthropogenic impact on marine organisms and on the ecosystems.</p> <p>Intended audience: biologists, hydrobiologists, ecologists, radiobiologists, biophysicists, oceanologists, geographers, scientists of other related specialties, graduate students, and students of relevant scientific profiles.</p> <p>The subscription index in the “<a title="Russian Press MBJ" href="https://www.pressa-rf.ru/cat/1/edition/e38872/" target="_blank" rel="noopener">Russian Press</a>” catalogue is Е38872.</p> </div> </div> A. O. Kovalevsky Institute of Biology of the Southern Seas of RAS, Sevastopol, Russian Federation en-US Marine Biological Journal 2499-9768 Computer methods for determining Mnemiopsis leidyi motility characteristics https://marine-biology.ru/mbj/article/view/364 <p>The paper considers capacities of common computer programs for analyzing the behavioral reactions to light of ctenophores, marine planktonic animals, under laboratory conditions. We showed that most image analysis programs do not automatically determine body contours of translucent objects, such as ctenophores. We developed a new method for determining basic motility characteristics of <em>Mnemiopsis leidyi</em> using ImageJ, Davinci Resolve video filters, and scripts written by the us. The developed method allows automatic calculating of such parameters as average and maximum speed, changes in direction of movement (locomotion vectors), and the percentage of time animals spend in certain regions of interest (ROI). The average speed of ctenophore movement was estimated in millimeter scale with high precision. The method is applicable for studying the behavior of various translucent marine animals. Computer scripts are available by request from the authors.</p> Iu. S. Baiandina O. N. Kuleshova Copyright (c) 2022 A. O. Kovalevsky Institute of Biology of the Southern Seas of RAS https://creativecommons.org/licenses/by-nc-sa/4.0 2022-11-29 2022-11-29 7 4 3 13 10.21072/mbj.2022.07.4.01 Heavy metals and arsenic in commercial fish of the Sea of Japan, Sea of Okhotsk, and Bering Sea: Current status (literature review) https://marine-biology.ru/mbj/article/view/362 <p>The paper summarizes literature data on the concentrations of toxic elements – As, Pb, Cd, and Hg – in commercial fish of the Far Eastern seas – the Sea of Japan, Sea of Okhotsk, and Bering Sea. According to the analysis carried out, main commercial facilities and fishery basins meet the sanitary and hygienic standards. However, the existence of impact natural areas in fish ranges and on the routes of their migration contributes to an increase in concentrations of toxic elements in fishery objects. In some cases, the values exceed the maximum permissible levels. In this regard, it is necessary to continue monitoring of toxic microelements in commercial facilities and fishery basins of the Far Eastern seas.</p> M. K. Gamov A. E. Ivanova E. K. Mironova V. Yu. Tsygankov Copyright (c) 2022 A. O. Kovalevsky Institute of Biology of the Southern Seas of RAS https://creativecommons.org/licenses/by-nc-sa/4.0 2022-11-29 2022-11-29 7 4 14 30 10.21072/mbj.2022.07.4.02 Growth dynamics of the benthic diatom Ardissonea crystallina (C. Agardh) Grunow, 1880 (Bacillariophyta) under copper ions effect https://marine-biology.ru/mbj/article/view/367 <p>Increasing anthropogenic load on coastal ecosystems of the Black Sea determines the need for regular assessing the state of planktonic and benthic communities. Planktonic microalgae contributing up to 20–25 % of global primary production are traditionally used as test objects; however, the contribution of microphytobenthos is comparable to that of phytoplankton. Benthic diatoms are close-associated with bottom substrate, and most of them are highly sensitive to the effect of technogenic pollutants accumulating in sediments. The changes in physiological indicators of benthic Bacillariophyta may objectively reflect the negative effect of various toxicants; accordingly, benthic diatoms can be used as test objects in the indirect assessment of the marine environment quality. We aimed to study the growth dynamics of abundance of clonal strain cells for a new biotesting object – the diatom <em>Ardissonea crystallina</em> (C. Agardh) Grunow, 1880 (Bacillariophyta) – under the effect of various CuSO<sub>4</sub>·5H<sub>2</sub>O concentrations during 10-day laboratory experiments. This species is widespread in the Black Sea sublittoral and highly sensitive to the effect of different technogenic pollutants, <em>inter alia</em> heavy metals. As shown, at copper ions concentrations of 32–128 μg·L<sup>−1</sup>, <em>A. crystallina</em> growth dynamics generally corresponds to the dose–response curve in a toxicological experiment. The correlation was found between a decrease in intensity of the culture growth and increase in toxicant concentration in the experimental medium. At copper ions concentration of 256–320 μg·L<sup>−1</sup>, the ratio of alive cells in the clonal strain decreases gradually from 62–66 % (the 1<sup>st</sup> day) to 34–37 % (the 10<sup>th</sup> day); the indicators of an increase in cell abundance in the clonal strain are characterized by a negative trend – from −0.01 (on the 2<sup>nd</sup> day) to −0.34 (on the 10<sup>th</sup> day). At Cu<sup>2+</sup> concentrations of 384 μg·L<sup>−1</sup> and higher, drastic inhibition and subsequent death of <em>A. crystallina</em> cells were revealed. At 448–1,024 μg·L<sup>−1</sup>, complete cell mortality was registered already on the 3<sup>rd</sup> day of the experiment. Statistical comparison of the ratio variability of <em>A. crystallina</em> alive cells and the specific growth in their abundance for the control and Cu<sup>2+</sup> concentrations of 64–128 μg·L<sup>−1</sup> showed as follows: at 32–128 μg·L<sup>−1</sup>, the differences between the mean values of the test indicators were significant (<em>P</em> = 0.002…0.020). At 256 μg·L<sup>−1</sup>, the changes in total abundance and alive cells ratio in the test culture significantly differ (<em>P</em> = 0.002…0.014) from those both at lower and higher copper concentrations. This fact allows to consider the toxicant level of 256 μg·L<sup>−1</sup> as a critical one for <em>A. crystallina</em>: its exceeding will result in a sharp increase in cell mortality. Based on the results obtained, this benthic diatom can be recommended for use as a suitable test object in toxicological experiments, as well as for monitoring and indirect environmental assessment of coastal water areas subjected to technogenic pollution.</p> E. L. Nevrova A. N. Petrov Copyright (c) 2022 A. O. Kovalevsky Institute of Biology of the Southern Seas of RAS https://creativecommons.org/licenses/by-nc-sa/4.0 2022-11-29 2022-11-29 7 4 31 45 10.21072/mbj.2022.07.4.03 Investigation of the long-wavelength threshold of spectral sensitivity in the gray seal Halichoerus grypus (Fabricius, 1791) https://marine-biology.ru/mbj/article/view/363 <p>In marine mammals, the absorption maxima of photopigments have a shift to the shorter-wavelength spectrum area compared to terrestrial mammals; this leads to a shift in the long-wavelength threshold of spectral sensitivity as well. In most publications focused on the investigation of long-wavelength sensitivity of marine mammals, only the absorption maxima of “red-sensitive” photopigments are given, but no data on maximum wavelengths of light emission that animals are able to perceive are provided. Therefore, this work was aimed at studying the long-wavelength thresholds of spectral sensitivity in a typical representative of earless seals – the gray seal <em>Halichoerus grypus</em> (Fabricius, 1791). During the experiment, a group of four gray seals was trained to press one of two buttons if a LED lamp located above it is switched on. In the lamp, there were groups of LEDs emitting monochromatic light in the wavelength range from 600 to 700 nm with a step of 10 nm and a luminous intensity of 0.5 cd. As shown, the lower perception threshold of light emission for the studied gray seals is light emission with a wavelength of 660 nm, and this confirms the data on the short-wavelength shift of the sensitivity peaks of photopigments in marine mammals. During prolonged exposure to extremely low-light conditions typical for the polar night, the long-wavelength perception threshold in the gray seals can increase from 660 to 670–680 nm.</p> M. V. Pakhomov A. A. Zaytsev Copyright (c) 2022 A. O. Kovalevsky Institute of Biology of the Southern Seas of RAS https://creativecommons.org/licenses/by-nc-sa/4.0 2022-11-29 2022-11-29 7 4 46 54 10.21072/mbj.2022.07.4.04 Suspended particulate matter as a biochemical barrier to heavy metals in marine farm areas (Sevastopol, the Black Sea) https://marine-biology.ru/mbj/article/view/365 <p>For recreational zones and marine farm areas, the investigation of chemical fluxes in coastal marine areas is of certain relevance. To study the role of suspended particulate matter in formation of biogeochemical barriers in marine farm areas, a method was proposed for estimating the fluxes of sedimentary self-purification of water from heavy metals (Co, Ni, Cu, Zn, Mo, Cd, and Pb) and arsenic (As). Based on the literature data on radioisotope dating of bottom sediments and the sedimentation rate, as well as considering our own estimates of the specific gravity of suspended particulate matter in water and concentrations of dissolved and suspended forms of heavy metals and As in the marine environment, the fluxes of biogeochemical self-purification of a marine farm water area from these trace elements were determined. As shown, the proposed methodological base is applicable for ecological regulation of coastal recreational zones in terms of pollution by trace elements.</p> N. V. Pospelova V. N. Egorov V. Yu. Proskurnin A. S. Priimak Copyright (c) 2022 A. O. Kovalevsky Institute of Biology of the Southern Seas of RAS https://creativecommons.org/licenses/by-nc-sa/4.0 2022-11-29 2022-11-29 7 4 55 69 10.21072/mbj.2022.07.4.05 Determination of control levels of radionuclides ensuring acceptable environmental risk in the Barents Sea water and bottom sediments https://marine-biology.ru/mbj/article/view/360 <p>To assess the radioecological situation, criteria were developed ensuring acceptable environmental risk – control levels of radionuclides in the components of the natural ecosystem. The method was applied to evaluate the control levels of technogenic radionuclides in the Barents Sea water and bottom sediments. If these levels are not exceeded, marine biota is considered protected from radioactive contamination. Local concentration factors of <sup>137</sup>Cs, <sup>90</sup>Sr, and <sup>239</sup>Pu in the Barents Sea biota were estimated using the data of long-term observations. Moreover, the reference organisms were selected – a fish (cod), mollusc (mussel), aquatic plant (fucus), and marine mammal (harp seal). The values of <sup>137</sup>Cs, <sup>239</sup>Pu, and <sup>90</sup>Sr concentration factors were, respectively, as follows (L·kg<sup>−1</sup>): in fish, 93, 262, and 12; in molluscs, 51, 1,180, and 21; in aquatic plants, 69, 732, and 19; and in marine mammals, 63, 222, and 14. The values of the water–sediment distribution coefficients of <sup>137</sup>Cs, <sup>239</sup>Pu, and <sup>90</sup>Sr were 426, 189,600, and 443 L·kg<sup>−1</sup>, respectively. For most radionuclides and the reference organisms from the Barents Sea, the values of radionuclide concentration differ from the global average reference values. For the period of 1992–2020, there is no pronounced temporal trend for the concentration factors of all technogenic radionuclides in the Barents Sea fish; this indicates the establishment of equilibrium in the distribution of radioactivity between the components of the Arctic marine ecosystem. The control levels of radionuclides were as follows: in the Barents Sea water (Bq·L<sup>−1</sup>), 115 for <sup>137</sup>Cs, 439 for <sup>90</sup>Sr, and 0.124 for <sup>239,240</sup>Pu; in the Barents Sea bottom sediments (kBq·kg<sup>−1</sup> fresh weight), 48.9 for <sup>137</sup>Cs, 194 for <sup>90</sup>Sr, and 23.6 for <sup>239,240</sup>Pu. The contamination index for both water and bottom sediments of the Barents Sea was calculated using the monitoring data and reference levels. In 2006–2020, its values were several orders of magnitude lower than 1 and did not tend to increase or decrease. In terms of marine biota protection, the main contributor to the contamination index for the Barents Sea water is <sup>239,240</sup>Pu (up to 75 %) while the main contributor to the contamination index for the Barents Sea bottom sediments is <sup>137</sup>Cs (up to 90 %). To date, the ratio of the contributions of technogenic radionuclides to the contamination index for the Barents Sea water and bottom sediments is stable.</p> N. A. Rosnovskaya A. I. Kryshev I. I. Kryshev Copyright (c) 2022 A. O. Kovalevsky Institute of Biology of the Southern Seas of RAS https://creativecommons.org/licenses/by-nc-sa/4.0 2022-11-29 2022-11-29 7 4 70 80 10.21072/mbj.2022.07.4.06 Phytoplankton primary production on the northeastern Sakhalin Island shelf in summer https://marine-biology.ru/mbj/article/view/361 <p>The eastern Sakhalin Island shelf is the area of high biological production. Its key peculiarity is the presence of a feeding area for the Okhotsk–Korean population of gray whales. We aimed at determining the features of the formation of primary production in this area; thereby, on 7–9 July, 2016, hydrochemical studies on the northeastern Sakhalin Island shelf were carried out. At each station, water was sampled from surface and near-bottom layers; then, concentrations of chlorophyll <em>a</em>, nitrates, and phosphates were measured. Moreover, at each station, depth profiling was conducted by a Sea-Bird SBE 19plus and a Rinko-Profiler. Those profilers were equipped with sensors for pressure, temperature, electrical conductivity, chlorophyll fluorescence, dissolved oxygen, turbidity, and photosynthetically active radiation. Assimilation number for phytoplankton was measured <em>in situ</em> by ARO1-USB Rinko dissolved oxygen sensors (JFE Advantech Co., Ltd.). Phytoplankton primary production in the photic layer was determined by the light model based on the representation of the photosynthetic light-response curve in the modified model of the non-rectangular hyperbola. Most intensively, the primary production occurred in the area affected by the Amur River. In the photic layer, the values of integral primary production varied within 1.57–11.17 g C·m<sup>−2</sup>·day<sup>−1</sup>. The distribution area of the modified highly productive water of the Amur River reached the traverse of the southern boundary of the Piltun Bay; there, it was limited by cold salty water which had risen due to the eddy structure from deeper horizons. The ratio of the production spent on the food supply formation for the Okhotsk–Korean population of gray whales was 1.9 % of the total production of the studied water area.</p> P. P. Tishchenko Copyright (c) 2022 A. O. Kovalevsky Institute of Biology of the Southern Seas of RAS https://creativecommons.org/licenses/by-nc-sa/4.0 2022-11-29 2022-11-29 7 4 81 97 10.21072/mbj.2022.07.4.07 Records of the brown alga Halosiphon tomentosus (Lyngbye) Jaasund (Phaeophyceae) in the South-Eastern Baltic Sea https://marine-biology.ru/mbj/article/view/366 <p>For the first time, <em>Halosiphon tomentosus</em> (Lyngb.) Jaasund (<em>Chorda tomentosa</em> Lyngb.) was recorded in the South-Eastern Baltic Sea in May–June 2016, in several locations of the Sambia Peninsula northern coast. The species was found in the upper horizon of sublittoral on boulders in assemblages with green and brown algae (<em>Ulva intestinalis</em>, <em>Ulva prolifera</em>, <em>Cladophora glomerata</em>, <em>Ectocarpus siliculosus</em>, <em>Pylaiella littoralis</em>, and sometimes <em>Pseudolithoderma subextensum</em> and <em>Hildenbrandia rubra</em>). The species was recorded in 2017–2018 as well. The length of thread-like thalli ranged 3–30 cm, with the mean value of (9.2 ± 2.3) cm. The mean biomass was of (73.3 ± 41.9) g·m<sup>−2</sup> in 2016 and (11 ± 8.8) g·m<sup>−2</sup> in 2018. The reasons for <em>H. tomentosus</em> occurrence in the South-Eastern Baltic and its absence in the adjacent sea areas require further research.</p> A. A. Volodina Copyright (c) 2022 A. O. Kovalevsky Institute of Biology of the Southern Seas of RAS https://creativecommons.org/licenses/by-nc-sa/4.0 2022-11-29 2022-11-29 7 4 98 102 10.21072/mbj.2022.07.4.08 On the anniversary of D. Sc. Vitaly Ryabushko https://marine-biology.ru/mbj/article/view/379 <p>On 15 February, 2022, D. Sc. Vitaly Ryabushko celebrated his 75<sup>th</sup> birthday. V. Ryabushko, the chief researcher of IBSS aquaculture and marine pharmacology department, is the author of more than 300 scientific publications, chairman of the specialized dissertation council in hydrobiology at IBSS, and academician of the Russian Ecological Academy, Petrovsky Academy of Sciences and Arts, and Crimean Academy of Sciences.</p> Collegues Copyright (c) 2022 A. O. Kovalevsky Institute of Biology of the Southern Seas of RAS https://creativecommons.org/licenses/by-nc-sa/4.0 2022-11-29 2022-11-29 7 4 103 104 On the anniversary of D. Sc. Larisa Ryabushko https://marine-biology.ru/mbj/article/view/380 <p>On 14 July, 2022, D. Sc. Larisa Ryabushko celebrated her 75<sup>th</sup> birthday. L. Ryabushko, the chief researcher of IBSS aquaculture and marine pharmacology department, is the author of about 220 scientific papers and academician of the Russian Ecological Academy and Petrovsky Academy of Sciences and Arts.</p> Collegue Copyright (c) 2022 A. O. Kovalevsky Institute of Biology of the Southern Seas of RAS https://creativecommons.org/licenses/by-nc-sa/4.0 2022-11-29 2022-11-29 7 4 105 106 Scientific activity of D. Sc., Prof. Ernest Samyshev (to his 85th birthday) https://marine-biology.ru/mbj/article/view/383 <div> <div>On 28 October, 2022, D. Sc., Prof. Ernest Samyshev celebrates his anniversary. He is the head of IBSS marine ecosystems functioning department, author of more than 270 scientific papers, and winner of the State Prize of Ukraine in Science and Technology (2007). His research interests cover hydrobiology, ecology, biocenology, anthropogenic transformation of marine ecosystems, and mariculture of fish and invertebrates.</div> </div> Collegues Copyright (c) 2022 A. O. Kovalevsky Institute of Biology of the Southern Seas of RAS https://creativecommons.org/licenses/by-nc-sa/4.0 2022-11-29 2022-11-29 7 4 107 109 Centenary of the research vessel “Persey” https://marine-biology.ru/mbj/article/view/378 <p>The research vessel “Persey” was constructed a hundred years ago, in 1922. It is the first Soviet ship designed for marine scientific research and the first vessel of the Knipovich Polar Research Institute of Marine Fisheries and Oceanography.</p> K. M. Sokolov T. E. Pashkova A. N. Benzik Copyright (c) 2022 A. O. Kovalevsky Institute of Biology of the Southern Seas of RAS https://creativecommons.org/licenses/by-nc-sa/4.0 2022-11-29 2022-11-29 7 4 110 111 In memoriam: Yuri Mordvinov (17.06.1937 – 18.09.2022) https://marine-biology.ru/mbj/article/view/382 <p>On 18 September, 2022, D. Sc. Yuri Mordvinov passed away. He studied the main patterns of formation and development of nekton adaptations in secondary aquatic vertebrates – reptiles, birds, and mammals – and published 70 scientific works.</p> Collegues Copyright (c) 2022 A. O. Kovalevsky Institute of Biology of the Southern Seas of RAS https://creativecommons.org/licenses/by-nc-sa/4.0 2022-11-29 2022-11-29 7 4 112 113