Determination of the growth rate and elimination of certain phytoplankton species and populations in the Sevastopol Bay (Black Sea)
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Abstract
The relevance of the study is determined by the key role of phytoplankton in the functioning of marine ecosystems, since unicellular algae carry out the primary production of organic matter in photosynthesis and form the first trophic link in the food chain. The tasks of the work are to determine the values of the net (apparent) growth rate of phytoplankton as a whole and of its constituent elements, including populations of certain species, and to study the possibility of estimating the rate of their actual growth and consumption based on regular short-period determinations of phytoplankton abundance and biomass in natural communities. For the determinations, we used both the previously obtained data of the decadal monitoring of the phytoplankton state in the coastal zone and the results of recent experiments. The decadal determinations performed during 2007 near the mouth of the Sevastopol Bay included measurements of phytoplankton abundance and biomass, as well as of chlorophyll a concentrations at intervals of several days, which made it possible to calculate the net specific biomass growth rate (apparent growth, k) according to its changes identified during this period. By comparing the abundance of certain species during short-period determinations, 29 values of the apparent growth were obtained for 9 mass species. The apparent growth, being the difference between actual growth and consumption, can be used to determine these values. Patterns linking the values of the apparent and actual growth of algae cells with their sizes were found in the experiments conducted by the dilution method. In our experiments, we adapted classical dilution method for the study of total phytoplankton to determine the functional parameters of certain species, which made it possible to find the linking patterns. Using the found patterns, we obtained 22 values of growth rate µ and consumption rate m for 7 mass phytoplankton species at the mouth of the Sevastopol Bay. Studies show that the growth rate of certain species of phytoplankton, whose cell volume is less then 1000 µm³, can reach values over 1 day−1. For algae with the cell volume exceeding 1500 µm³, the values of µ approach the values of k, and the values of m approach zero. For this group, the actual growth rate µ is taken to be equal to the apparent growth rate k, and the rate of consumption m is taken to be zero. In fact, in the natural population, small species are predominantly consumed in the upper illuminated layer, while the larger ones sink to the bottom or into deep horizons. This means that primarily phytoplankton populations with small cells are consumed, while populations with larger cells are not consumed. However, this conclusion is not consistent with the rates of apparent growth measured in situ, which include both positive and negative values for all populations, regardless of cell size. This contradiction is explained by the fact, that in the experiment, large cells dying off and sinking to the bottom of the vessel are taken into account on a par with living cells, and it hides the effect of elimination.
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References
Антипова Н. Л., Загоренко Г. Ф. К вопросу об определении суточной продукции некоторых видов Байкальского фитопланктона // Известия Биолого-географического научно-исследовательского института при Иркутском государственном университете. 1971. Т. 25, № 1. С. 20–29. [Antipova N. L., Zagorenko G. F. K voprosu ob opredelenii sutochnoi produktsii nekotorykh vidov Baikal’skogo fitoplanktona. Izvestiya Biologo-geograficheskogo nauchno-issledovatel’skogo instituta pri Irkutskom gosudarstvennom universitete, 1971, vol. 25, no. 1, pp. 20–29. (in Russ.)]
Богоров В. Г. Планктон Мирового океана. Москва : Наука, 1974. 320 с. [Bogorov V. G. Plankton Mirovogo okeana. Moscow: Nauka, 1974, 320 p. (in Russ.)]
Водоросли : справочник. Киев : Наукова думка, 1989. 608 с. [Vodorosli : spravochnik. Kiev: Naukova dumka, 1989, 608 p. (in Russ.)]
Зенкевич Л. А. Биология морей СССР. Москва : Изд-во АН СССР, 1963. 740 с. [Zenkevich L. A. Biologiya morei SSSR. Moscow: Izd-vo AN SSSR, 1963, 740 p. (in Russ.)]
Киселев И. А. Методы исследования планктона // Жизнь пресных вод СССР / под ред. Е. Н. Павловского, В. И. Жадина. Москва ; Ленинград : Изд-во АН СССР, 1956. Т. 4. С. 183–270. [Kiselev I. A. Metody issledovaniya planktona. In: Zhizn’ presnykh vod SSSR / E. N. Pavlovskii, V. I. Zhadin (Eds). Moscow ; Leningrad: Izd-vo AN SSSR, 1956, vol. 4, pp. 183–270. (in Russ.)]
Кондратьева Т. М. Определение суточной продукции фитопланктона в Севастопольской бухте // Первичная продукция морей и внутренних вод / под ред. Г. Г. Винберга. Минск : Изд-во Мин. высшего и среднего спец. образования БССР, 1961. С. 71–82. [Kondrat’eva T. M. Opredelenie sutochnoi produktsii fitoplanktona v Sevastopol’skoi bukhte. In: Pervichnaya produktsiya morei i vnutrennikh vod / G. G. Vinberg (Ed.). Minsk: Izd-vo Min. vysshego i srednego spets. obrazovaniya BSSR, 1961, pp. 71–82. (in Russ.)]
Неврова Е. Л. Таксономическое разнообразие и структура таксоцена бентосных диатомовых (Bacillariophyta) в Севастопольской бухте (Чёрное море) // Морской экологический журнал. 2013. Т. 12, № 3. С. 55–67. [Nevrova E. L. Taxonomic diversity and structure of benthic diatom taxocene (Bacillariophyta) at Sevastopol Bay (the Black Sea). Morskoj ekologicheskij zhurnal, 2013, vol. 12, no. 3, pp. 55–67. (in Russ.)]. https://repository.marine-research.ru/handle/299011/1296
Сеничева М. И. Сезонная динамика численности, биомассы и продукции фитопланктона Севастопольской бухты // Экология моря. 1980. Вып. 1. С. 3–11. [Senicheva M. I. Seasonal dynamics of the phytoplankton number, biomass and production in the Sevastopol Bay. Ekologiya morya, 1980, iss. 1, pp. 3–11. (in Russ.)]. https://repository.marine-research.ru/handle/299011/2722
Сеничева М. И. Динамика популяции Skeletonema costatum (Grev.) в Севастопольской бухте // Экология моря. 1980. Вып. 1. С. 11–15. [Senicheva M. I. Dynamic of Skeletonema costatum population in the Sevastopol Bay. Ekologiya morya, 1980, iss. 1, pp. 11–15. (in Russ.)]. https://repository.marine-research.ru/handle/299011/2723
Сеничева М. И. Видовое разнообразие, сезонная и межгодовая изменчивость микроводорослей в планктоне у берегов Крыма // Микроводоросли Черного моря: проблемы сохранения биоразнообразия и биотехнологического использования / под ред. Ю. Н. Токарева, З. З. Финенко, Н. В. Шадрина ; НАН Украины, Институт биологии южных морей. Севастополь : ЭКОСИ-Гидрофизика, 2008. С. 5–17. [Senicheva M. I. Species diversity, seasonal and interannual variability of microalgae in plankton off the coast of the Crimea. In: The Black Sea Microalgae: Problems of Biodiversity Preservation and Biotechnological Usage / Yu. N. Tokarev, Z. Z. Finenko, N. V. Shadrin (Eds) ; NAS of Ukraine, Institute of Biology of the Southern Seas. Sevastopol: ECOSI-Gidrofizika, 2008, pp. 5–17. (in Russ.)]. https://repository.marine-research.ru/handle/299011/5521
Сорокин Ю. И. К методике концентрирования проб фитопланктона // Гидробиологический журнал. 1979. Т. 15, № 2. С. 71–76. [Sorokin Yu. I. K metodike kontsentrirovaniya prob fitoplanktona. Gidrobiologicheskij zhurnal, 1979, vol. 15, no. 2, pp. 71–76. (in Russ.)]
Стельмах Л. В., Куфтаркова Е. А., Бабич И. И. Сезонная изменчивость скорости роста фитопланктона в прибрежных водах Чёрного моря (район Севастополя) // Морской экологический журнал. 2009. Т. 8, № 1. С. 67–80. [Stelmakh L. V., Kuftarkova E. A., Babich I. I. Seasonal variations of phytoplankton growth rate in coastal waters of the Black Sea (near Sevastopol). Morskoj ekologicheskij zhurnal, 2009, vol. 8, no. 1, pp. 67–80. (in Russ.)]. https://repository.marine-research.ru/handle/299011/1001
Стельмах Л. В., Бабич И. И., Родионова Н. Ю. Основные структурно-функциональные характеристики фитопланктона поверхностных вод западной части Черного моря в летний период // Системы контроля окружающей среды. 2012. Вып. 17. С. 171–176. [Stelmakh L. V., Babich I. I., Rodionova N. Yu. Main structural and functional characteristics of phytoplankton in surface waters of the western Black Sea in summer. Sistemy kontrolya okruzhayushchei sredy, 2012, iss. 17, pp. 171–176. (in Russ.)]
Стельмах Л. В., Мансурова И. И., Акимов А. И. Культуры динофитовых водорослей Чёрного моря: экспериментальные исследования и практическое значение // Экосистемы, их оптимизация и охрана. 2014. Вып. 11. С. 260–266. [Stelmakh L. V., Mansurova I. M., Akimov A. I. Cultures of the Black Sea dinoflagellates: Experimental studies and practical significance. Ekosistemy, ikh optimizatsiya i okhrana, 2014, iss. 11, pp. 260–266. (in Russ.)]
Суханова И. Н. Концентрирование фитопланктона в пробе // Современные методы количественной оценки распределения морского планктона. Москва : Наука, 1983. С. 97–105. [Sukhanova I. N. Phytoplankton concentration in the sample. In: Sovremennye metody kolichestvennoi otsenki raspredeleniya morskogo planktona. Moscow: Nauka, 1983, pp. 97–105. (in Russ.)]
Чмыр В. Д., Сеничева М. И., Ли Р. И., Щербатенко Л. С. Кинетика структуры и видимой скорости роста фитопланктона у побережья Севастополя // Экобиологические проблемы Азово-Черноморского региона и комплексное управление биологическими ресурсами : тез. науч.-практ. конф., Севастополь, 28–30 сент. 2015 г. Севастополь, 2015. С. 171–174. [Tchmyr V. D., Senicheva M. I., Lee R. I., Scherbatenko L. S. Kinetics of the structure and apparent growth rate of phytoplankton off the coast of Sevastopol. In: Ecobiological problems of the Azov – Black Sea region and integrated management of biological resources : tez. nauch.-prakt. konf., Sevastopol, 28–30 Sept., 2015. Sevastopol, 2015, pp. 171–174. (in Russ.)]
Чмыр В. Д., Сеничева М. И., Ли Р. И., Щербатенко Л. С. Сообщество фитопланктона: чередование и скорость роста популяций // Морские биологические исследования: достижения и перспективы : в 3-х т. : сб. материалов Всерос. науч.-практ. конф. с междунар. участием, приуроч. к 145-летию Севастопольской биологической станции, Севастополь, 19–24 сент. 2016 г. / под общ. ред. А. В. Гаевской. Севастополь, 2016. Т. 2. С. 469–472. [Tchmyr V. D., Senicheva M. I., Lee R. I., Scherbatenko L. S. Phytoplankton community: Alternation and growth rate of populations. In: Morskie biologicheskie issledovaniya: dostizheniya i perspektivy : v 3-kh t. : sb. materialov Vseros. nauch.-prakt. konf. s mezhdunar. uchastiem, priuroch. k 145-letiyu Sevastopol’skoi biologicheskoi stantsii, Sevastopol, 19–24 Sept., 2016 / A. V. Gaevskaya (Ed.). Sevastopol, 2016, vol. 2, pp. 469–472. (in Russ.)]
Behrenfeld M. J. Abandoning Sverdrup’s Critical Depth Hypothesis on phytoplankton blooms. Ecology, 2010, vol. 91, no. 4, pp. 977–989. https://doi.org/10.1890/09-1207.1
Calbet A., Saiz E. Effects of trophic cascades in dilution grazing experiments from artificial saturated feeding responses to positive slopes. Journal of Plankton Research, 2013, vol. 35, iss. 6, pp. 1183–1191. https://doi.org/10.1093/plankt/fbt067
Gran H. H., Braarud T. A quantitative study on the phytoplankton of the Bay of Fundy and the Gulf of Maine (including observations on hydrography, chemistry and turbidity). Journal of the Biological Board of Canada, 1935, vol. 1, no. 5, pp. 279–467. https://doi.org/10.1139/f35-012
Landry M. R., Hassett R. P. Estimating the grazing impact of marine micro-zooplankton. Marine Biology, 1982, vol. 67, iss. 3, pp. 283–288. https://doi.org/10.1007/BF00397668
Landry M. R., Haas L. W., Fagerness V. L. Dynamics of microplankton communities: Experiments in Kaneohe Bay, Hawaii. Marine Ecology Progress Series, 1984, vol. 16, no. 1–2, pp. 127–133. http://dx.doi.org/10.3354/meps016127
Landry M. R., Brown S. L., Rii Y. M., Selph K. E., Bidigare R. R., Yang E. J., Simmons M. P. Depth-stratified phytoplankton dynamics in Cyclone Opal, a subtropical mesoscale eddy. Deep Sea Research Part II: Topical Studies in Oceanography, 2008, vol. 55, iss. 10–13, pp. 1348–1359. https://doi.org/10.1016/j.dsr2.2008.02.001
Lewis W. M. Jr. Net growth rate through time as an indicator of ecological similarity among phytoplankton species. Ecology, 1977, vol. 58, no. 1, pp. 149–157.
Li Q. P., Franks P. J. S., Landry M. R., Goericke R., Taylor A. G. Modeling phytoplankton growth rates and chlorophyll to carbon ratios in California coastal and pelagic ecosystems. Journal of Geophysical Research – Biogeosciences, 2010, vol. 115, iss. G4, article ID G04003. http://dx.doi.org/10.1029/2009jg001111
Sverdrup H. U. On conditions for the vernal blooming of phytoplankton. Journal du Conseil / Conseil Permanent International pour l’Exploration de la Mer, 1953, vol. 18, iss. 3, pp. 287–295.
