Growth of Isochrysis galbana Parke, 1949 (Haptophyta) under mixotrophic conditions using salicylic acid
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Abstract
The effect of salicylic acid at different concentrations on growth dynamics of Isochrysis galbana Parke, 1949 in the batch culture was estimated. The cultivation was carried out in monoculture. The rise in algal biomass was evaluated by an increase in cell abundance (cells were counted in each experiment in the Goryaev chamber in triplicate under a light microscope). The experiments lasted for 7 days. As found, salicylic acid at concentrations from 2.8 × 10−7 to 5.6 × 10−7 mol·L−1 had a stimulating effect on the growth dynamics of I. galbana cells, compared with the control group. The maximum cell growth in culture was recorded with the addition of 2.8 × 10−7 mol·L−1 of salicylic acid, and the specific growth rate at a given concentration on the 7th day of the experiment was 1.2 times higher than in the control group. Biochemical parameters of I. galbana culture with salicylic acid added (2.8 × 10−7 mol·L−1) during 7 days of the experiment were estimated in comparison with parameters of the control group. In the experimental group, the maximum protein content was noted on the 7th day of the experiment. A rise was 76.9% compared to the initial value. As shown, the maximum increase in the content of lipids and carbohydrates in the experimental group occurred on the 5th day. A rise in the values of these indicators was 41.7 and 87%, respectively. Chlorophyll content increased throughout the entire experiment both in the control and experimental groups, and the highest value was registered for the experimental group.
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References
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