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Markina Zh. V., Popik A. Yu. Interactions of the diatom algae Pseudo-nitzschia hasleana and Thalassiosira pseudonana in the mixed culture. Marine Biological Journal, 2023, vol. 8, no. 3, pp. 47-61. https://doi.org/10.21072/mbj.2023.08.3.04

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Abstract

Representatives of the genus Pseudo-nitzschia (Bacillariophyta) cause blooms in different areas of the World Ocean. Therefore, it is necessary to know their ecological features, including the way those interact with other species of unicellular algae. Moreover, for rapid identification of these algae in the environment, a certain technique is needed. Thus, we assessed the dynamics of cell abundance for Pseudo-nitzschia hasleana and Thalassiosira pseudonana in mono- and mixed cultures by their direct counting in a Nageotte chamber. Temperature curves of chlorophyll a fluorescence obtained by laser-induced fluorescence in a temperature chamber were also analyzed. The experiments lasted for 14 days. As shown, P. hasleana had different effect on T. pseudonana depending on initial abundance of T. pseudonana. At initial concentration of 0.8 × 104 cells·mL−1, a pronounced stimulation of the growth of this diatom occurred. At initial concentrations of 1.6 × 104 and 3.2 × 104 cells·mL−1, T. pseudonana growth was inhibited. In the mixed culture, T. pseudonana remained at the stationary growth phase, while in a monoculture, the population entered the dying phase by the 14th day of the experiment. T. pseudonana had an inhibitory effect on P. hasleana growth. The experiment with P. hasleana and T. pseudonana co-cultivation showed as follows: chlorophyll a fluorescence of the mixture is more affected by the microalga with much higher concentration. The fluorescent signal of two separately cultivated monocultures can potentially be used to search for these cultures in a mixture.

Authors

Zh. V. Markina

researcher, PhD

https://orcid.org/0000-0001-7135-1375

https://elibrary.ru/author_items.asp?id=251096

A. Yu. Popik

researcher, PhD

https://orcid.org/0000-0001-9306-6196

https://elibrary.ru/author_items.asp?id=670551

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Funding

Работа выполнена при финансовой поддержке гранта РНФ № 21-74-30004.

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