Bioluminescent bacteria of the Black Sea and Sea of Azov
##plugins.themes.ibsscustom.article.main##
Google Scholar
##plugins.themes.ibsscustom.article.details##
Abstract
The aim of the present study was to isolate bioluminescent strains from the northern Black Sea and Sea of Azov, analyze their morphological and biochemical characteristics, and identify them based on 16S rRNA, recA, and gyrB gene sequences. Nine isolates were isolated from hydrobionts, and twelve, from seawater. Results of biochemical and molecular genetic identification revealed that isolated luminous strains represent the genera Vibrio, Aliivibrio, and Photobacterium. All five cultivated luminescent strains isolated from water and hydrobionts of the Sea of Azov belong to the species Photobacterium leiognathi. Cultivated luminous bacteria of the Black Sea are assigned to the genera Aliivibrio and Vibrio. The genus Aliivibrio is represented by two Aliivibrio fischeri strains related to various hydrobionts. Fourteen strains of the genus Vibrio belong to the species Vibrio campbellii, V. jasicida, V. harveyi, V. owensii, and V. aquamarinus sp. nov. Thus, it was shown that taxonomic composition of the cultivated luminescent bacteria differs greatly in the Black Sea and Sea of Azov.
Authors
References
Ast J. C., Cleenwerck I., Engelbeen K., Urbanczyk H., Thompson F. L., De Vos P., Dunlap P. V. Photobacterium kishitanii sp. nov., a luminous marine bacterium symbiotic with deep-sea fishes. International Journal of Systematic and Evolutionary Microbiology, 2007, vol. 57, iss. 9, pp. 2073–2078. https://doi.org/10.1099/ijs.0.65153-0
Ast J. C., Urbanczyk H., Dunlap P. V. Multi-gene analysis reveals previously unrecognized phylogenetic diversity in Aliivibrio. Systematic and Applied Microbiology, 2009, vol. 32, iss. 6, pp. 379–386. https://doi.org/10.1016/j.syapm.2009.04.005
Baumann P., Schubert R. H. W. Family II. Vibrionaceae Veron 1956, 5245AL. In: Bergey’s Manual of Systematic Bacteriology / D. H. Bergey, N. R. Krieg, J. G. Holt (Eds). Baltimore ; London : Williams & Wilkins, 1984, vol. 1, pp. 516–517.
Baumstark-Khan C., Rabbow E., Rettberg P., Horneck G. The combined bacterial Lux-Fluoro test for the detection and quantification of genotoxic and cytotoxic agents in surface water: Results from the “Technical Workshop on Genotoxicity Biosensing”. Aquatic Toxicology, 2007, vol. 85, iss. 3, pp. 209–218. https://doi.org/10.1016/j.aquatox.2007.09.003
Cano-Gómez A., Goulden E. F., Owens L., Høj L. Vibrio owensii sp. nov., isolated from cultured crustaceans in Australia. FEMS Microbiology Letters, 2010, vol. 302, iss. 2, pp. 175–181. https://doi.org/10.1111/j.1574-6968.2009.01850.x
Chugunova E. A., Mukhamatdinova R., Sazykina M., Dobrynin A., Sazykin I., Karpenko A., Mirina E., Zhuravleva M., Karchava S., Burilov A. Synthesis of new ‘hybrid’ compounds based on benzofuroxans and aminoalkylnaphthalimides. Chemical Biology & Drug Design, 2016, vol. 87, iss. 4, pp. 626–634. https://doi.org/10.1111/cbdd.12685
Deryabin D. G. Bakterial’naya biolyuminestsentsiya: fundamental’nye i prikladnye aspekty. Moscow : Nauka, 2009, 246 p. (in Russ.)
Dunlap P. V., Urbanczyk H. Luminous bacteria. In: The Prokaryotes / E. Rosenberg, E. F. DeLong, S. Lory, E. Stackebrandt, F. Thompson (Eds). Berlin ; Heidelberg : Springer, 2013, pp. 495–528. https://doi.org/10.1007/978-3-642-30141-4_75
Farmer III J. J., Michael Janda J. Vibrionaceae. In: Bergey’s Manual of Systematics of Archaea and Bacteria. Hoboken, New Jersey : John Wiley & Sons : Bergey’s Manual Trust, 2015. https://doi.org/10.1002/9781118960608.fbm00212
Farmer III J. J., Michael Janda J., Brenner F. W., Cameron D. N., Birkhead K. M. Vibrio. In: Bergey’s Manual of Systematics of Archaea and Bacteria. Hoboken, New Jersey : John Wiley & Sons : Bergey’s Manual Trust, 2015. https://doi.org/10.1002/9781118960608.gbm01078
Gomez-Gil B., Thompson F. L., Thompson C. C., Swings J. Vibrio rotiferianus sp. nov., isolated from cultures of the rotifer Brachionus plicatilis. International Journal of Systematic and Evolutionary Microbiology, 2003, vol. 53, iss. 1, pp. 239–243. https://doi.org/10.1099/ijs.0.02430-0
Ivask A., Green T., Polyak B., Mor A., Kahru A., Virta M., Marks R. Fibre-optic bacterial biosensors and their application for the analysis of bioavailable Hg and As in soils and sediments from Aznalcollar mining area in Spain. Biosensors and Bioelectronics, 2007, vol. 22, iss. 7, pp. 1396–1402. https://doi.org/10.1016/j.bios.2006.06.019
Katsev A. M. Utilities of luminous bacteria from the Black Sea. Applied Biochemistry and Microbiology, 2002, vol. 38, iss. 2, pp. 189–192. https://doi.org/10.1023/A:1014327020286
Katsev A. M., Makemson J. Identification of luminescent bacteria isolated from the Black and Azov seas. Uchenye zapiski Tavricheskogo natsional’nogo universiteta imeni V. I. Vernadskogo. Seriya Biologiya. Khimiya, 2006, vol. 19 (58), no. 4, pp. 111–116.
Kovalenko S. I., Nosulenko I. S., Voskoboynik A. Yu., Berest G. G., Antipenko L. N., Antipenko A. N., Katsev A. M. Novel N-aryl(alkaryl)-2-[(3-R-2-oxo-2H-[1,2,4]triazino[2,3-c]quinazoline-6-yl)thio]acetamides: Synthesis, cytotoxicity, anticancer activity, COMPARE analysis and docking. Medicinal Chemistry Research, 2013, vol. 22, no. 6, pp. 2610–2632. https://doi.org/10.1007/s00044-012-0257-x
Kumar S., Stecher G., Li M., Knyaz C., Tamura K. MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Molecular Biology and Evolution, 2018, vol. 35, iss. 6, pp. 1547–1549. https://doi.org/10.1093/molbev/msy096
Kuryanov V. O., Chupakhina T. A., Shapovalova A. A., Katsev A. M., Chirva V. Ya. Glycosides of hydroxylamine derivatives: I. Phase transfer synthesis and the study of the influence of glucosaminides of isatine 3-oximes on bacterial luminescence. Russian Journal of Bioorganic Chemistry, 2011, vol. 37, iss. 2, pp. 231–239. https://doi.org/10.1134/S1068162011020105
Labella A. M., Arahal D. R., Castro D., Lemos M. L., Borrego J. J. Revisiting the genus Photobacterium: Taxonomy, ecology and pathogenesis. International Microbiology, 2017, vol. 20, iss. 1, pp. 1–10. https://doi.org/10.2436/20.1501.01.280
Lucena T., Ruvira M. A., Arahal D. R., Macian M. C., Pujalte M. J. Vibrio aestivus sp. nov. and Vibrio quintilis sp. nov., related to Marisflavi and Gazogenes clades, respectively. Systematic and Applied Microbiology, 2012, vol. 35, iss. 7, pp. 427–431. https://doi.org/10.1016/j.syapm.2012.08.002
Maligina V. Yu., Katsev A. M. Luminous bacteria from the Black Sea and the Sea of Azov. Ekologiya morya, 2003, vol. 64, pp. 18–23. (in Russ.). https://repository.marine-research.ru/handle/299011/4587
Moi I. M., Roslan N. N., Leow A. T. C., Mohamad Ali M. S., Rahman R. N. Z. R. A., Rahimpour A., Sabri S. The biology and the importance of Photobacterium species. Applied Microbiology and Biotechnology, 2017, vol. 101, iss. 11, pp. 4371–4385. https://doi.org/10.1007/s00253-017-8300-y
Niu S., Wang S., Shi C., Zhang S. Studies on the fluorescence fiber-optic DNA biosensor using p-hydroxyphenylimidazo[f]1,10-phenanthroline ferrum(III) as indicator. Journal of Fluorescence, 2008, vol. 18, iss. 1, pp. 227–235. https://doi.org/10.1007/s10895-007-0266-1
Patent 2358009 RU, MPK C12N 1/20, C12Q 1/04. Isolation Method of Bioluminescent Bacteria / M. A. Sazykina, I. E. Tsybulskii, K. S. Abrosimova, Southern Federal University. No. 2007114379/13, zayvl. 16.04.2007, opubl. 10.06.2009. Bul. no. 16. (in Russ.)
Patent 2368658 RU, MPK C12N 1/20, C12Q 1/04. Nutrient Medium for Bioluminescent Bacteria Cultivation / M. A. Sazykina, I. E. Tsybulskii, K. S. Abrosimova, Southern Federal University. No. 2007114380/13, zayvl. 16.04.2007, opubl. 27.09.2009. Bul. no. 27. (in Russ.)
Saitou N., Nei M. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, 1987, vol. 4, iss. 4, pp. 406–425. https://doi.org/10.1093/oxfordjournals.molbev.a040454
Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, 1977, vol. 74, no. 12, pp. 5463–5467. https://doi.org/10.1073/pnas.74.12.5463
Sazykin I. S., Sazykina M. A., Khammami M. I., Khmelevtsova L. E., Kostina N. V., Trubnik R. G. Distribution of polycyclic aromatic hydrocarbons in surface sediments of lower reaches of the Don River (Russia) and their ecotoxicologic assessment by bacterial lux-biosensors. Environmental Monitoring and Assessment, 2015, vol. 187, no. 5, art. no. 277 (12 p.). https://doi.org/10.1007/s10661-015-4406-9
Sazykin I. S., Sazykina M. A., Khmelevtsova L. E., Mirina E. A., Kudeevskaya E. M., Rogulin E. A., Rakin A. V. Biosensor-based comparison of the ecotoxicological contamination of the wastewaters of Southern Russia and Southern Germany. International Journal of Environmental Science and Technology, 2016, vol. 13, iss. 3, pp. 945–954. http://doi.org/10.1007/s13762-016-0936-0
Sönmez A. Y., Sazykina M., Bilen S., Gültepe N., Sazykin I., Khmelevtsova L. E., Kostina N. V. Assessing contamination in sturgeons grown in recirculating aquaculture system by lux-biosensors and metal accumulation. Fresenius Environmental Bulletin, 2016, vol. 25, no. 4, pp. 1028–1037.
Taga M. E., Bassler B. L. Chemical communication among bacteria. Proceedings of the National Academy of Sciences, 2003, vol. 100, no. suppl_2, pp. 14549–14554. https://doi.org/10.1073/pnas.1934514100
Thompson F. L., Iida T., Swings J. Biodiversity of Vibrios. Microbiology and Molecular Biology Reviews, 2004, vol. 68, no. 3, pp. 403–431. https://doi.org/10.1128/MMBR.68.3.403-431.2004
Thompson F. L., Hoste B., Vandemeulebroecke K., Swings J. Reclassification of Vibrio hollisae as Grimontia hollisae gen. nov., comb. nov. International Journal of Systematic and Evolutionary Microbiology, 2003, vol. 53, iss. 5, pp. 1615–1617. https://doi.org/10.1099/ijs.0.02660-0
Thyssen A., Ollevier F. Photobacterium. In: Bergey’s Manual of Systematics of Archaea and Bacteria. Hoboken, New Jersey : John Wiley & Sons : Bergey’s Manual Trust, 2015. https://doi.org/10.1002/9781118960608.gbm01076
Tsybulskii I. E., Sazykina M. A. New biosensors for assessment of environmental toxicity based on marine luminescent bacteria. Applied Biochemistry and Microbiology, 2010, vol. 46, iss. 5, pp. 505–510. https://doi.org/10.1134/S0003683810050078
Urbanczyk H., Ast J. C., Dunlap P. V. Phylogeny, genomics, and symbiosis of Photobacterium. FEMS Microbiology Reviews, 2011, vol. 35, iss. 2, pp. 324–342. https://doi.org/10.1111/j.1574-6976.2010.00250.x
Urbanczyk H., Ast J. C., Higgins M. J., Carson J., Dunlap P. V. Reclassification of Vibrio fischeri, Vibrio logei, Vibrio salmonicida and Vibrio wodanis as Aliivibrio fischeri gen. nov., comb. nov., Aliivibrio logei comb. nov., Aliivibrio salmonicida comb. nov. and Aliivibrio wodanis comb. nov. International Journal of Systematic and Evolutionary Microbiology, 2007, vol. 57, iss. 12, pp. 2823–2829. https://doi.org/10.1099/ijs.0.65081-0
Wang Y., Zhang X.-H., Yu M., Wang H., Austin B. Vibrio atypicus sp. nov., isolated from the digestive tract of the Chinese prawn (Penaeus chinensis O’sbeck). International Journal of Systematic and Evolutionary Microbiology, 2010, vol. 60, iss. 11, pp. 2517–2523. https://doi.org/10.1099/ijs.0.016915-0
Yoshizawa S., Karatani H., Wada M., Yokota A., Kogure K. Aliivibrio sifiae sp. nov., luminous marine bacteria isolated from seawater. Journal of General and Applied Microbiology, 2010a, vol. 56, iss. 6, pp. 509–518. https://doi.org/10.2323/jgam.56.509
Yoshizawa S., Tsuruya Y., Fukui Y., Sawabe T., Yokota A., Kogure K., Higgins M., Carson J., Thompson F. L. Vibrio jasicida sp. nov., a member of the Harveyi clade, isolated from marine animals (packhorse lobster, abalone and Atlantic salmon). International Journal of Systematic and Evolutionary Microbiology, 2012, vol. 62, iss. Pt_8, pp. 1864–1870. https://doi.org/10.1099/ijs.0.025916-0
Yoshizawa S., Wada M., Kita-Tsukamoto K., Ikemoto E., Yokota A., Kogure K. Vibrio azureus sp. nov., a luminous marine bacterium isolated from seawater. International Journal of Systematic and Evolutionary Microbiology, 2009a, vol. 59, iss. 7, pp. 1645–1649. https://doi.org/10.1099/ijs.0.004283-0
Yoshizawa S., Wada M., Kita-Tsukamoto K., Yokota A., Kogure K. Photobacterium aquimaris sp. nov., a luminous marine bacterium isolated from seawater. International Journal of Systematic and Evolutionary Microbiology, 2009b, vol. 59, iss. 6, pp. 1438–1442. https://doi.org/10.1099/ijs.0.004309-0
Yoshizawa S., Wada M., Yokota A., Kogure K. Vibrio sagamiensis sp. nov., luminous marine bacteria isolated from sea water. Journal of General and Applied Microbiology, 2010b, vol. 56, iss. 6, pp. 499–507. https://doi.org/10.2323/jgam.56.499
Zheng H., Liu L., Lu Y., Long Y., Wang L., Ho K.-P., Wong K.-Y. Rapid determination of nanotoxicity using luminous bacteria. Analytical Science, 2010, vol. 26, iss. 1, pp. 125–128. https://doi.org/10.2116/analsci.26.125
