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Dantsyuk N. V., Chelebieva E. S., Minyuk G. S. Working collection of carotenogenic microalgae living cultures of A. O. Kovalevsky Institute of Biology of the Southern Seas. Marine Biological Journal, 2021, vol. 6, no. 4, pp. 3-18. https://doi.org/10.21072/mbj.2021.06.4.01

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Abstract

The article contains information on the specialized working collection of carotenogenic microalgae maintained by the staff of the animal physiology and biochemistry department of A. O. Kovalevsky Institute of Biology of the Southern Seas of RAS (IBSS). The collection was established within the framework of IBSS scientific and applied research to study the mechanisms of stress tolerance in eurybiontic and extremophilic single-celled phototrophs and to identify commercially significant sources of highly valuable ketocarotenoids of astaxanthin group used for medicine and food production. The collection contains 44 microalgal strains of various taxonomic and ecological specialization with a pronounced ability to hypersynthesize secondary carotenoids and lipids under extreme conditions (drying, nutrient starvation, high-intensity illumination, extreme temperature and salinity, effect of toxicants, etc.). The main ways to replenish the fund are direct exchange of carotenogenic species with leading Russian and foreign collections of microalgae and own field sampling in the Black Sea areas of Crimea and Caucasus. The majority of strains in the collection represent two orders of the class Chlorophyceae: Chlamydomonadales (25 strains) and Sphaeropleales (15 strains), since the phenomenon of secondary carotenogenesis is widespread in these orders. Out of them, inhabitants of ephemeral freshwater ponds predominate, as well as aerophilic and soil microalgae. All strains are maintained under controlled conditions on agarized mineral media as pure cultures. Description of the collection accession includes the following data: a) current taxonomic status of the species verified according to updated information from corresponding collections and algological databases, namely AlgaeBase and NCBI Taxonomy Browser; b) species basionym and known synonyms; c) date and source of the strain deposition; d) author’s surname, geographic location, and biotope, from which the strain was isolated; e) accession number of sequences associated with the strain in NCBI (if any); and f) nutrient medium, on which the strain is maintained in the IBSS collection. The significance of the collection for morphological, biological, physiological, and biochemical studies of growth, secondary carotenogenesis, and biotechnological potential in green microalgae is discussed.

Authors

N. V. Dantsyuk

junior researcher

https://elibrary.ru/author_items.asp?id=757663

E. S. Chelebieva

researcher, PhD

https://orcid.org/0000-0002-7662-2573

https://elibrary.ru/author_items.asp?id=757658

G. S. Minyuk

leading researcher, PhD

https://orcid.org/0000-0001-7182-3299

https://elibrary.ru/author_items.asp?id=195277

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Funding

This work was carried out within the framework of IBSS state research assignment “Investigation of mechanisms of controlling production processes in biotechnological complexes with the aim of developing scientific foundations for production of biologically active substances and technical products of marine genesis” (No. 121030300149-0).

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