Halotolerance limits of the Black Sea representative of the genus Entomoneis Ehrenberg, 1845 (Bacillariophyta)
##plugins.themes.bootstrap3.article.main##
##plugins.themes.bootstrap3.article.details##
Abstract
The genus Entomoneis Ehrenberg, 1845 is quite rich in species. Underestimated diversity of this genus requires its deeper morphological and molecular study, as well as an investigation of ecological and physiological characteristics of its species – specifically, their tolerance limits to environmental factors. Considering the distribution of Entomoneis species in water bodies with various salinity, we aimed at studying the tolerance limits and determining optimal salinity for vegetative growth and sexual reproduction of the diatom Entomoneis sp. from the Black Sea. We used reproductively compatible clonal cultures isolated from samples taken on the Crimean and Turkish Black Sea coasts. For Entomoneis sp. clone 7.0906-D, the nucleotide sequence of the rbcL gene was obtained; it is presented in the GenBank database under the number MT424817. Morphologically, the studied species resembles E. paludosa; according to molecular data, it is far from it. In accordance with its ecological and physiological characteristics, this species is a marine one. According to published material available, E. paludosa, unlike Entomoneis sp., inhabits brackish, slightly saline, and even fresh water bodies. Experiments on halotolerance show the following: the Black Sea clones of Entomoneis sp. are viable in a range of at least 40 ‰ (8 to 48 ‰). A salinity range of the medium within which Entomoneis sp. revealed sexual reproduction is much narrower – 18 to 36 ‰. Optimal salinity values for vegetative growth and sexual reproduction were determined (27.4 and 26.4 ‰, respectively); those turned out to be higher in both cases than the values in the natural habitat of this species. As salinity of the medium increased, Entomoneis sp. initial cells resulting from sexual reproduction tended to decrease in size.
Authors
References
Давидович О. И. Влияние солёности на вегетативный рост и половое воспроизведение диатомовой водоросли Tabularia tabulata (C. A. Agardh) Snoeijs // Актуальные проблемы биоразнообразия и природопользования : материалы Всероссийской научно-практической конференции, Керчь, 26 сентября – 1 октября 2017 г. Симферополь : АРИАЛ. 2017. С. 38 –43. [Davidovich O. I. Vliyanie solenosti na vegetativnyi rost i polovoe vosproizvedenie diatomovoi vodorosli Tabularia tabulata (C. A. Agardh) Snoeijs. In: Aktual’nye problemy bioraznoobraziya i prirodopol’zovaniya : materialy Vserossiiskoi nauchno-prakticheskoi konferentsii, Kerch, 26 September – 1 October, 2017. Simferopol : ARIAL, 2017, pp. 38 –43. (in Russ.)]
Караева Н. И., Джафарова С. К. Экспериментальные исследования полигалобных Bacillariophyta в связи с солёностью среды // Альгология. 1993. Т. 3, № 2. С. 97 –105. [Karaeva N. I., Dzhafarova S. K. Experimental investigations of polygalobic Bacillariophyta in connection with medium salinity. Al’gologiya, 1993, vol. 3, no. 2, pp. 97 –105. (in Russ.)]
Куликовский М. С., Глущенко А. М., Генкал С. И., Кузнецова И. В. Определитель диатомовых водорослей России. Ярославль : Филигрань, 2016. 804 с. [Kulikovskiy M. S., Glushchenko A. M., Genkal S. I., Kuznetsova I. V. Opredelitel’ diatomovykh vodoroslei Rossii. Yaroslavl : Filigran’, 2016, 804 p. (in Russ.)]
Полякова С. Л., Давидович О. И., Подунай Ю. А., Давидович Н. А. Модификация среды ESAW, используемой для культивирования морских диатомовых водорослей // Морской биологический журнал. 2018. Т. 3, № 2. С. 73 –80. [Polyakova S. L., Davidovich O. I., Podunai Yu. A., Davidovich N. A. Modification of the ESAW culture medium used for cultivation of marine diatoms. Morskoj biologicheskij zhurnal, 2018, vol. 3, no. 2, pp. 73 –78. (in Russ.)]. https://doi.org/10.21072/mbj.2018.03.2.06
Рябушко Л. И. Микроводоросли бентоса Чёрного моря (чек-лист, синонимика, комментарий). Севастополь : ЭКОСИ-Гидрофизика, 2006. 143 с. [Ryabushko L. I. Microalgae of the Black Sea Benthos (Check-list, Synonyms, Comment). Sevastopol : EKOSI-Gidrofizika, 2006, 143 p. (in Russ.)]
AlgaeBase. World-wide electronic publication, National University of Ireland, Galway / Guiry M. D., Guiry G. M. (Eds) : [site], 2020. URL: http://www.algaebase.org [accessed: 27.05.2020].
Andersen R. A., Berges J. A., Harrison P. J., Watanabe M. M. Recipes for freshwater and seawater media. In: Algal Culturing Techniques. Amsterdam : Elsevier Academic Press, 2005, pp. 429 –538.
Bagmet V. B., Abdullin Sh. R., Kuluev B. R., Davidovich O. I., Davidovich N. A. The effect of salinity on the reproduction rate of Nitzschia palea (Kützing) W. Smith (Bacillariophyta) clones. Russian Journal of Ecology, 2017, vol. 48, iss. 3, pp. 287 –289. https://doi.org/10.1134/S1067413617030043
Brand L. E. The salinity tolerance of forty-six marine phytoplankton isolates. Estuarine, Coastal and Shelf Science, 1984, vol. 18, iss. 5, pp. 543 –556. https://doi.org/10.1016/0272-7714(84)90089-1
Dalu T., Taylor J. C., Richoux N. B., Froneman W. A re-examination of the type material of Entomoneis paludosa (W. Smith) Reimer and its morphology and distribution in African waters. Fottea, 2015, vol. 15, iss. 1, pp. 1 –25. https://doi.org/10.5507/fot.2015.002
Davidovich N. A., Davidovich O. I. Salinity optima for vegetative growth and sexual reproduction of the diatom Toxarium undulatum. Morskoj biologicheskij zhurnal, 2020, vol. 5, no. 1, pp. 20 –28. https://doi.org/10.21072/mbj.2020.05.1.03
Liu B., Williams D. M., Ector L. Entomoneis triundulata sp. nov. (Bacillariophyta), a new freshwater diatom species from Dongting Lake, China. Cryptogamie, Algologie, 2018, vol. 39, iss. 2, pp. 239 –253. https://doi.org/10.7872/crya/v39.iss2.2018.239
Mejdandžić M., Bosak S., Nakov T., Ruck E., Orlić S., Gligora Udovič M., Peharec Štefanić P., Špoljarić I., Mršić G., Ljubešić Z. Morphological diversity and phylogeny of the diatom genus Entomoneis (Bacillariophyta) in marine plankton: Six new species from the Adriatic Sea. Journal of Phycology, 2018, vol. 54, iss. 2, pp. 275 –298. https://doi.org/10.1111/jpy.12622
Morant-Manceau A., Nhung Nguyen T. L., Pradier E., Tremblin G. Carbonic anhydrase activity and photosynthesis in marine diatoms. European Journal of Phycology, 2007, vol. 42, iss. 3, pp. 263 –270. https://doi.org/10.1080/09670260701425522
Nagai S., Imai I. The effect of salinity on the size of initial cells during vegetative cell enlargement of Coscinodiscus wailesii (Bacillariophyta) in culture. Diatom Research, 1999, vol. 14, iss. 2, pp. 337 –342. https://doi.org/10.1080/0269249X.1999.9705475
Rech M., Mouget J.-L., Morant-Manceau A., Rosa P., Tremblin G. Long-term acclimation to UV radiation: Effects on growth, photosynthesis and carbonic anhydrase activity in marine diatoms. Botanica Marina, 2005, vol. 48, iss. 5, pp. 407 –420. https://doi.org/10.1515/bot.2005.054
Round F. E., Crawford R. M., Mann D. G. The Diatoms: Biology and Morphology of the Genera. Cambridge : Cambridge University Press, 1990, 747 p. https://doi.org/10.1017/S0025315400059245
Ryabushko L. I., Lishaev D. N., Kovrigina N. K. Species diversity of epilithon diatoms and the quality of the waters of the Donuzlav Gulf ecosystem (Crimea, the Black Sea). Diversity, 2019, vol. 11, iss. 7, art. no. 114 (12 p.). https://doi.org/10.3390/d11070114
Trobajo R., Rovira L., Mann D. G., Cox E. J. Effects of salinity on growth and on valve morphology of five estuarine diatoms. Phycological Research, 2011, vol. 59, iss. 2, pp. 83 –90. https://doi.org/10.1111/j.1440-1835.2010.00603.x
Weckstrom K., Juggins S. Coastal diatom –environment relationships from the Gulf of Finland, Baltic Sea. Journal of Phycology, 2006, vol. 42, iss. 1, pp. 21 –35. https://doi.org/10.1111/j.1529-8817.2006.00166.x