Reproductive biology and the life cycle of the diatom Nitzschia cf. thermaloides inhabiting mud volcanoes of Crimea
##plugins.themes.bootstrap3.article.main##
##plugins.themes.bootstrap3.article.details##
Abstract
In biology, it is important to study the ability of organisms to exist under extreme conditions, to which the phase of the life cycle, related to sexual reproduction and the possibility to leave the next generation, is especially sensitive. Nitzschia cf. thermaloides Hustedt was found in high abundance in samples from puddles and lakes formed in the areas of action of mud volcanoes of the Bulganak mud volcano field (Kerch Peninsula, Crimea). Individual clones were isolated from the samples by the micropipette technique and introduced into a culture; their crossing made it possible to initiate heterothallic sexual reproduction. The aim of this work was to study reproductive biology and the life cycle of the alga from a biotope with extremely high levels of irradiance, temperature, and salinity. For the first time for this species, a description of the sexual process corresponding to IB2a type according to Geitler classification is given. The cardinal points have been defined, which represent critical cell sizes that determine the transition from one phase of the life cycle to another. The full range of cell sizes is given, from the initial ones that appeared as a result of sexual reproduction to the smallest ones observed when kept in the culture. The main postulates of the life-cycle theory are considered allowing to analyze the natural population of the alga. The population of N. cf. thermaloides was found to exist in the sampling site for a long time and to be represented by cells in all phases of the life cycle.
Authors
References
Абдуллин Ш. Р., Багмет В. Б. Миксотрофия цианобактерий и водорослей в условиях пещер // Журнал общей биологии. 2016. Т. 77, № 1. С. 54–62. [Abdullin Sh. R., Bagmet V. B. Myxotrophy of cyanobacteriae and algae inhabiting caves. Zhurnal obshchei biologii, 2016, vol. 77, no. 1, pp. 54–62. (in Russ.)]
Давидович Н. А., Давидович О. И., Подунай Ю. А. Коллекция культур диатомовых водорослей Карадагской научной станции (Крым) // Морской биологический журнал. 2017. Т. 2, № 1. С. 18–28. [Davidovich N. A., Davidovich O. I., Podunay Yu. A. Diatom culture collection of the Karadag scientific station (Crimea). Morskoj biologicheskij zhurnal, 2017, vol. 2, no. 1, pp. 18–28. (in Russ.)]. https://doi.org/10.21072/mbj.2017.02.1.03
Каюкова Е. П. Химический состав жидкой фазы грязевых сопок северо-восточной части Керченского полуострова (Восточный Крым) // Геология, геоэкология, эволюционная география : коллективная монография. Т. 17 / под ред. Е. М. Нестерова, В. А. Снытко. Санкт-Петербург : Изд-во РГПУ им. А. И. Герцена, 2018. С. 186–190. [Kayukova E. P. Khimicheskii sostav zhidkoi fazy gryazevykh sopok severo-vostochnoi chasti Kerchenskogo poluostrova (Vostochnyi Krym). In: Geologiya, geoekologiya, evolyutsionnaya geografiya : collective monograph. Vol. 17 / E. M. Nesterov, V. A. Snytko (Eds). Saint Petersburg : Izd-vo RGPU im. A. I. Gertsena, 2018, pp. 186–190. (in Russ.)]
Куришко В. А., Месяц И. А., Тердовидов А. С. Гидрогеология грязевого вулканизма Керченского полуострова // Геологический журнал. 1968. Т. 8, вып. 1. С. 49–59. [Kurishko V. A., Mesyats I. A., Terdovidov A. S. Gidrogeologiya gryazevogo vulkanizma Kerchenskogo poluostrova. Geologicheskii zhurnal, 1968, vol. 8, iss. 1, pp. 49–59. (in Russ.)]
Матюнина В. А. Химический состав рассолов Булганакского и Мало-Тарханского сопочных полей (Восточный Крым) // Материалы Международного молодёжного научного форума «Ломоносов-2019» / отв. ред. И. А. Алешковский, А. В. Андриянов, Е. А. Антипов. Москва : МАКС Пресс, 2019. С. 1–2. [Matyunina V. A. Khimicheskii sostav rassolov Bulganakskogo i Malo-Tarkhanskogo sopochnykh polei (Vostochnyi Krym) // Materialy Mezhdunarodnogo molodezhnogo nauchnogo foruma “Lomonosov-2019” / I. A. Aleshkovsky, A. V. Andriyanov, E. A. Antipov (Eds). Moscow : MAKS Press, 2019, pp. 1–2. (in Russ.)]. URL: https://lomonosov-msu.ru/archive/Lomonosov_2019/6data/15896/92330_uid343668_report.pdf [accessed: 11.11.2020].
Неврова Е. Л., Шадрин Н. В. Донные диатомовые водоросли солёных озёр Крыма // Морской экологический журнал. 2005. Т. 4, № 4. С. 61–71. [Nevrova E. L., Shadrin N. V. Benthic diatoms in Crimean saline lakes. Morskoj ekologicheskij zhurnal, 2005, vol. 4, no. 4, pp. 61–71. (in Russ.)]. https://repository.marine-research.ru/handle/299011/821
Никулина Т. В. Водоросли горячих источников Курильских островов (Россия) // Альгология. 2010. Т. 20, № 3. С. 334–356. [Nikulina T. V. Biodiversity of algae of hot springs from Kuril Islands (Russia). Al’gologia, 2010, vol. 20, no. 3, pp. 334–356. (in Russ.)]. https://doi.org/10.1615/InterJAlgae.v12.i4.10
Полякова С. Л., Давидович О. И., Подунай Ю. А., Давидович Н. А. Модификация среды ESAW, используемой для культивирования морских диатомовых водорослей // Морской биологический журнал. 2018. Т. 3, № 2. С. 73–80. [Polyakova S. L., Davidovich O. I., Podunay Yu. A., Davidovich N. A. Modification of the ESAW culture medium used for cultivation of marine diatoms. Morskoj biologicheskij zhurnal, 2018, vol. 3, no. 2, pp. 73–80. (in Russ.)]. https://doi.org/10.21072/mbj.2018.03.2.06
Рощин А. М. Жизненные циклы диатомовых водорослей. Киев : Наукова думка, 1994. 171 с. [Roshchin A. M. Zhiznennye tsikly diatomovykh vodoroslei. Kyiv : Naukova dumka, 1994, 171 p. (in Russ.)]
Рябушко Л. И., Бондаренко А. В. Микроводоросли грязевого вулкана Булганакского сопочного поля Крымского полуострова // Морской биологический журнал. 2020. Т. 5, № 1. С. 64–77. [Ryabushko L. I., Bondarenko A. V. Microalgae of mud volcano of the Bulganak sopochnoe field on the Crimean Peninsula. Morskoj biologicheskij zhurnal, 2020, vol. 5, no. 1, pp. 64–77. (in Russ.)]. https://doi.org/10.21072/mbj.2020.05.1.07
Сеничева М. И., Губелит Ю. И., Празукин А. В., Шадрин Н. В. Фитопланктон гиперсолёных озёр Крыма // Микроводоросли Чёрного моря: проблемы сохранения биоразнообразия и биотехнологического использования / под ред. Ю. Н. Токарева, 3. 3. Финенко, Н. В. Шадрина ; НАН Украины, Институт биологии южных морей. Севастополь : ЭКОСИ-Гидрофизика, 2008. С. 93–99. [Senicheva M. I., Gubelit Yu. I., Prazukin A. V., Shadrin N. V. Fitoplankton gipersolenykh ozer Kryma // The Black Sea Microalgae: Problems of Biodiversity Preservation and Biotechnological Usage / Yu. N. Tokarev, Z. Z. Finenko, N. V. Shadrin (Eds) ; NAS of Ukraine, Institute of Biology of the Southern Seas. Sevastopol : EKOSI-Gidrofizika, 2008, pp. 93–99. (in Russ.)]. https://repository.marine-research.ru/handle/299011/5521
Armbrust E. V., Berges J. A., Bowler Ch., Green B. R., Martinez D., Putnam N. H., Zhou Sh., Allen A. E., Apt K. E., Bechner M., Brzezinski M. A., Chaal B. K., Chiovitti A., Davis A. K., Demarest M. S., Detter J. Ch., Glavina T., Goodstein D., Hadi M. Z., Hellsten U., Hildebrand M., Jenkins B. D., Jurka J., Kapitonov V. V., Kröger N., Lau W. W. Y., Lane T. W., Larimer F. W., Lippmeier J. C., Lucas S., Medina M., Montsant A., Obornik M., Parker M. Sch., Palenik B., Pazour G. J., Richardson P. M., Rynearson T. A., Saito M. A., Schwartz D. C., Thamatrakoln K., Valentin K., Vardi A., Wilkerson F. P., Rokhsar D. S. The genome of the diatom Thalassiosira pseudonana: Ecology, evolution, and metabolism. Science, 2004, vol. 306, iss. 5693, pp. 79–86. https://doi.org/10.1126/science.1101156
Drebes G. Sexuality. In: The Biology of Diatoms / D. Werner (Ed.). Oxford : Blackwell Scientific Publications, 1977, pp. 250–283. (Botanical Monographs ; vol. 13).
Chepurnov V. A., Mann D. G., Sabbe K., Vyverman W. Experimental studies on sexual reproduction in diatoms. International Review of Cytology, 2004, vol. 237, pp. 91–154. https://doi.org/10.1016/S0074-7696(04)37003-8
Davidovich N. A. Species-specific sizes and size range of sexual reproduction in diatoms. In: Proceedings of the 16th International Diatom Symposium, Greece, 25 August – 1 September, 2000 / A. Economou-Amilli (Ed.). Greece : University of Athens, 2000, pp. 191–196.
Gastineau R., Davidovich N. A., Hallegraeff G. M. Reproduction in microalgae. In: Reproductive Biology of Plants / K. G. Ramawat, J.-M. Mérillon, K. R. Shivanna (Eds). Boca Raton : CRC Press, 2014, pp. 1–28. https://doi.org/10.1201/b16535
Geitler L. Auxosporenbildung und Systematik bei pennaten Diatomeen und die Cytologie von Cocconeis-Sippen. Österreichische Botanische Zeitschrift, 1973, vol. 122, no. 5, pp. 299–321.
Mann D. G. Why didn’t Lund see sex in Asterionella? A discussion of the diatom life cycle in nature. In: Algae and the Aquatic Environment: Contributions in Honour of J. W. G. Lund / F. E. Round (Ed.). Bristol, UK : Biopress Ltd., 1988, pp. 384–412.
Montsant A., Jabbari K., Maheswari U., Bowler C. Comparative genomics of the pennate diatom Phaeodactylum tricornutum. Plant Physiology, 2005, vol. 137, iss. 2, pp. 500–513. https://doi.org/10.1104/pp.104.052829
Nakov T., Beaulieu J. M., Alverson A. J. Accelerated diversification is related to life history and locomotion in a hyperdiverse lineage of microbial eukaryotes (diatoms, Bacillariophyta). New Phytologist, 2018, vol. 219, iss. 1, pp. 462–473. https://doi.org/10.1111/nph.15137
Peletier H., Gieskes W. W. C., Buma A. G. J. Ultraviolet-B radiation resistance of benthic diatoms isolated from tidal flats in the Dutch Wadden Sea. Marine Ecology Progress Series, 1996, vol. 135, pp. 163–168. https://doi.org/10.3354/meps135163
Poulíčková A., Mann D. G. Diatom sexual reproduction and life cycles. In: Diatoms: Fundamentals and Applications / J. Seckbach, R. Gordon (Eds). Hoboken, New Jersey : Wiley ; Salem, Massachusetts : Scrivener Publishing LLC, 2019, pp. 245–272. https://doi.org/10.1002/9781119370741.ch11
Round F. E., Crawford R. M., Mann D. G. The Diatoms. Biology and Morphology of the Genera. Cambridge, UK : Cambridge University Press, 1990, 747 p.