Variability of nucleotide sequences in two gene markers (COI and H3) of Litorogammarus karadagiensis (Grintsov, 2009) (Amphipoda, Gammaridae) and the systematics of the genus Litorogammarus
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Abstract
We investigated intra- and interspecific variability of mitochondrial DNA cytochrome c oxidase subunit I gene (COI) and nuclear DNA histone H3 gene in Litorogammarus representatives. Our results indicate that histone H3 molecular marker is not suitable for species identification in this genus due to its insufficient sequence variability. The taxonomy of the studied species was refined based on the analysis of COI gene marker. Comprehensive analysis of genetic distances (p-distances) performed involving ABGD and ASAP algorithms suggested that COI gene fragment is a diagnostic marker for species identification in Litorogammarus. Phylogenetic reconstruction based on four methods and analysis of the resulting gene trees for COI revealed close genetic relationship between L. karadagiensis and L. glareophilus. For these two species, we found several common morphological features: the presence of plumose setae on the mandibles, first maxillae, maxillipeds, and on the articles of the first and second gnathopods. The combined use of molecular and morphological data proved to be highly valuable for species identification and for resolving taxonomic issues within this group of amphipods.
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References
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