Estimation of the production characteristics and chemical composition of Scenedesmus rubescens with varying availability of mineral substrate
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Biotechnology of microalgae is a promising field for obtaining renewable sources of biomass rich in proteins, lipids, and pigments; this makes the search for optimal cultivation conditions highly relevant. Representatives of the genus Scenedesmus Meyen, 1829 feature rapid growth, resistance to environmental changes, and high content of biologically active compounds, which determines their potential for use in the food, pharmaceutical, and energy industries. The aim of the study was to analyze the growth performance and photosynthetic pigment content in a culture of Scenedesmus rubescens (P. J. L. Dangeard) E. Kessler, M. Schafer, C. Hummer, A. Kloboucek & V. A. R. Huss, 1997 on three types of mineral nutrient media: complete Tamiya medium, its half-strength modification (Tamiya ½), and Bold’s basal medium (BBM) (variants No. 1, 2, and 3, respectively). In the experiment, S. rubescens was cultured for 16 days under continuous illumination and additional carbon dioxide supply. On BBM and Tamiya medium, a high specific growth rate (µ) was recorded: 0.48 and 0.49 day−1, respectively. When using Tamiya ½ nutrient medium, S. rubescens specific growth rate was lower: 0.33 day−1. Linear growth on all three media was observed up to the 4th day. During this period, the cell density of S. rubescens in experimental variants No. 1 and 3 increased nearly 7-fold, and in variant No. 2, by 3.7 times. The maximum productivity of S. rubescens for the experimental variants No. 1, 2, and 3 was 0.35, 0.18, and 0.31 g dry weight·L−1·day−1, respectively, with no morphological changes in cells. Significant differences were revealed in the biochemical and kinetic growth characteristics of S. rubescens cultured on the three nutrient media. High concentrations of chlorophyll a and b were recorded in cells grown on Tamiya medium and BBM. The obtained results indicate very similar growth characteristics of S. rubescens for variants No. 1 and 3 during the exponential and linear growth phases. This suggests that cultivation on BBM can provide productivity and accumulation of valuable compounds comparable to those reached on Tamiya medium.
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