Влияние суточной гипоксии на функциональные показатели гемоцитов Anadara kagoshimensis (Tokunaga, 1906)
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Аннотация
Марикультурные хозяйства традиционно расположены в прибрежных участках, где моллюски могут подвергаться воздействию гипоксии. Культивирование в условиях дефицита кислорода приводит к снижению темпов роста, вспышкам заболеваний на фермах и массовой гибели моллюсков. Методом проточной цитометрии исследовано влияние краткосрочной гипоксии на функциональные показатели гемоцитов анадары (Anadara kagoshimensis). Контрольную группу содержали при 6,7–6,8 мг O2·л−1, опытную — при 0,4–0,5 мг O2·л−1. Экспозиция — 24 часа. Содержание кислорода в воде снижали, продувая её газообразным азотом. В гемолимфе моллюска на основании относительного размера и относительной гранулярности идентифицировано две группы гемоцитов: гранулоциты (эритроциты) и агранулоциты (амёбоциты). Эритроциты — преобладающий тип клеток в гемолимфе A. kagoshimensis: их доля составила более 90 % от общего числа клеток. Суточная гипоксия не привела к изменениям клеточного состава гемолимфы анадары. Способность к продукции активных форм кислорода и уровень смертности гемоцитов моллюсков экспериментальной группы также остались на уровне контрольных значений. Результаты проведённого исследования свидетельствуют о толерантности анадары к условиям острого дефицита кислорода.
Авторы
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