Korolev V. G. Molecular bases of the effect of low doses of radiation. Marine Biological Journal, 2020, vol. 5, no. 3, pp. 23-29. https://doi.org/10.21072/mbj.2020.05.3.03



By definition, low doses are minimum doses of a damaging agent, in particular radiation, causing a recorded biological effect. The problem of exposure to low doses of radiation is being discussed in scientific literature for decades, but there is still no generally accepted conclusion concerning the existence of some features of the effect of low doses in contrast to that of acute exposure. This is due to the fact as follows: if being fixed, these effects have a weak expression and can be easily criticized. The second important aspect of this problem is that biological effects are mainly described phenomenologically in literature, without deciphering their molecular causes. In recent years, a number of articles appeared in which the authors, when studying exposure to low doses of DNA-tropic agents, show that postreplication repair (in particular, its error-free branch) plays a key role in these effects. In the laboratory of eukaryotic genetics of Petersburg Nuclear Physics Institute named by B. P. Konstantinov, it was possible to isolate unique yeast mutants with a disrupted branch of error-free postreplication repair. A study of the processes of eliminating DNA damage with minimal deviations of their number from a spontaneous level made it possible to explain at the molecular level the differences in cell response to low doses from acute exposure.


V. G. Korolev

head of the laboratory, D. Sc.




Иванов Е. Л., Федорова И. В., Ковальцова С. В. Выделение и характеристика новых мутантов дрожжей Saccharomyces cerevisiae с повышенной спонтанной мутабельностью // Генетика. 1992. Т. 28. С. 47–55. [Ivanov E. L., Fedorova I. V., Kovaltzova S. V. Isolation and characterization of new mutants of the yeast Saccharomyces cerevisiae with increased spontaneous mutability. Genetika, 1992, vol. 28, pp. 47–55. (in Russ.)]

Baynton K., Bresson-Roy A., Fuchs R. P. P. Analysis of damage tolerance pathways in Saccharomyces cerevisiae: A requirement for Rev3 DNA polymerase in translesion synthesis. Molecular and Cellular Biology, 1998, vol. 18, iss. 2, pp. 960–966. https://doi.org/10.1128/MCB.18.2.960

Bridges B. A., Munson R. J. Mutagenesis in Escherichia coli: Evidence for the mechanism of base change mutation by ultraviolet radiation in a strain deficient in excision-repair. Proceedings of the Royal Society B: Biological Sciences, 1968, vol. 171, iss. 1023, pp. 213–226. https://doi.org/10.1098/rspb.1968.0065

Friedberg E. C. Suffering in silence: The tolerance of DNA damage. Nature Reviews Molecular Cell Biology, 2005, vol. 6, iss. 12, pp. 943–953. https://doi.org/10.1038/nrm1781

Gangavarapu V., Santa Maria S. R., Prakash S., Prakash L. Requirement of replication checkpoint protein kinases Mec1/Rad53 for postreplication repair in yeast. mBio, 2011, vol. 2, iss. 3, e00079-11. https://dx.doi.org/10.1128/mBio.00079-11

Ganesan A. K. Persistence of pyrimidine dimers during post-replication repair in ultraviolet light-irradiated Escherichia coli K12. Journal of Molecular Biology, 1974, vol. 87, iss. 1, pp. 103–119. https://doi.org/10.1016/0022-2836(74)90563-4

Hishida T., Kubota Y., Carr A. V., Iwasaki H. RAD6RAD18RAD5-pathway-dependent tolerance to chronic low-dose ultraviolet light. Nature, 2009, vol. 457, pp. 612–615. https://doi.org/10.1038/nature07580

Huang D., Piening B. D., Paulovich A. G. The preference for error-free postreplication repair in Saccharomyces cerevisiae exposed to low-dose methyl methanesulfonate is cell cycle dependent. Molecular and Cellular Biology, 2013, vol. 33, iss. 8, pp. 1515–1527. https://doi.org/10.1128/MCB.01392-12

Ivanov E. L., Kovaltzova S. V., Korolev V. G. Saccharomyces cerevisiae mutants with enhanced induced mutation and altered mitotic gene conversion. Mutation Research / Fundamental and Molecular Mechanisms of Mutagenesis, 1989, vol. 213, iss. 2, pp. 105–115. https://doi.org/10.1016/0027-5107(89)90141-3

Pages V., Santa Maria S. R., Prakash L., Prakash S. Role of DNA damage-induced replication checkpoint in promoting lesion bypass by translesion synthesis in yeast. Genes & Development, 2009, vol. 23, iss. 12, pp. 1438–1449. https://doi.org/10.1101/gad.1793409

Prakash L. Characterization of postreplication repair in Saccharomyces cerevisiae and effects of rad6, rad18, rev3, and rad52 mutations. Molecular and General Genetics MGG, 1981, vol. 184, iss. 3, pp. 471–478. https://doi.org/10.1007/bf00352525

Zhang H., Lawrence C. W. The error-free component of the RAD6/RAD18 DNA damage tolerance pathway of budding yeast employs sister-strand recombination. Proceedings of the National Academy of Sciences of the United States of America, 2005, vol. 102, iss. 44, pp. 15954–15959. https://doi.org/10.1073/pnas.0504586102



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