Markina Zh., Zinov A., Orlova T. Experience of growing the microalga Tisochrysis lutea (Haptophyta) under conditions of a Labfors bioreactor for the production of carotenoids and neutral lipids. Marine Biological Journal, 2024, vol. 9, no. 1, pp. 70-75. https://doi.org/10.21072/mbj.2024.09.1.05



The results of the experiment on the use of a Labfors 5 Lux LED flat panel bioreactor (Infors HT, Switzerland) for Tisochrysis lutea (Haptophyta) cultivation are presented. During the three-week study, growth and size structure of the microalga population were assessed, and the content of chlorophyll a, carotenoids, and neutral lipids was estimated. The highest cell abundance, 5.3 × 104 cells·mL−1, was recorded at the end of the experiment, on the 21st day. An increase in the proportion of 4–6-μm cells was registered on the 11th day. The maximum accumulation of carotenoids occurred on the 18th day (3.3 mg·L−1), and neutral lipids (Nile Red fluorescence was of 5.3 × 106), on the 14th–21st day. As revealed, Labfors 5 Lux LED flat panel bioreactor can be successfully used for cultivation of the microalga T. lutea.


Zh. Markina

researcher, PhD



A. Zinov



T. Orlova

chief researcher, PhD




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The work was carried out within the framework of NSCMB FEB RAS state research assignment “Dynamics of marine ecosystems, adaptation of marine organisms and communities to changes in the environment” (No. 121082600038-3) and with the financial support of the Russian Science Foundation grant No. 21-74-30004.



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