Опыт выращивания микроводоросли Tisochrysis lutea (Haptophyta) в условиях биореактора Labfors для продуцирования каротиноидов и нейтральных липидов
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Аннотация
Приведены результаты эксперимента по использованию биореактора панельного типа Labfors 5 Lux LED flat panel (Infors HT, Швейцария) для культивирования Tisochrysis lutea (Haptophyta). В ходе трёхнедельного исследования оценивали рост и размерную структуру популяции микроводоросли, содержание хлорофилла a, каротиноидов и нейтральных липидов. Максимальная численность клеток, 5,3 × 104 кл.·мл−1, зафиксирована к концу эксперимента, на 21-е сутки. Увеличение доли клеток размером 4–6 мкм регистрировали на 11-е сутки опыта. Наибольшее накопление каротиноидов происходило на 18-е сутки эксперимента (3,3 мг·л−1), нейтральных липидов (флуоресценция Nile Red составляла 5,3 × 106) — на 14–21-е сутки. Выявлено, что биореактор панельного типа Labfors 5 может быть успешно использован для культивирования микроводоросли T. lutea.
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