Количественное определение массовой доли суммарного йода в морских гидробионтах спектрофотометрическим методом

Abstract

The essence of the method is the mineralization of biomass in the presence of KOH, the oxidation of inorganic iodine compounds with bromine to iodates with the removal of excess bromine with formate. Iodates in an acidic medium are reduced with iodide to elemental iodine, which is extracted with chloroform and determined by light absorption in the range of 502–510 nm. The lower limit of the determined content is 10⁻⁶ g. To increase sensitivity, a 10% alcohol solution of potassium iodide is added to the chloroform fraction. After the formation of potassium triiodide, iodine is determined by light absorption in the region of 225 or 325 nm. The lower limit of the determined content is 10⁻⁹ g.

Keywords: microorganisms, iodine-organic substances