Суточная динамика накопления полифенолов в клетках Fucus vesiculosus и Ascophyllum nodosum в период полярного дня на побережье Баренцева моря

Abstract

Text Polyphenols are a group of secondary metabolites that protect the plant body from ultraviolet radiation, participate in plant metabolism, and also have therapeutic properties. Studying the dynamics of polyphenol accumulation in brown algae such as Fucus vesiculosus and Ascophyllum nodosum is of considerable interest for understanding the mechanisms of adaptation of these organisms to changing environmental conditions. The purpose of the work: to identify daily changes in the content of polyphenols in the cells of brown algae Fucus vesiculosus and Ascophyllum nodosum growing on the coast of the Barents Sea, to analyze the dependence of their accumulation on light and temperature, and to determine the role of endogenous rhythms and external factors in the regulation of polyphenol metabolism in brown algae cells. The study was conducted in July 2022-2023 on the coast of the Barents Sea, both in the natural environment and in the laboratory. The Folin-Denis spectrophotometric method was used to analyze the polyphenol content. The significant daily dynamics of polyphenols is shown, the main changes in the content of substances occur during the daytime and during dehumidification. When immersed, the concentration of polyphenols does not change. The concentration of polyphenols depends on a combination of light level, temperature, and alternating immersion/dehumidification periods. When in constant conditions (laboratory), the daily rhythm of changes in the concentration of polyphenols is not preserved, which may indicate a reduced role of endogenous rhythms and the predominant role of environmental factors in the regulation of polyphenol synthesis. The data obtained will expand information on the physiological and biochemical features of fucus algae in the Arctic zone, as well as on the mechanisms of plant adaptation to growing in the tidal zone of the seas. They can also be used in the development of algae aquaculture technology to obtain raw materials with desired properties.

Keywords: polyphenols, Fucus vesiculosus, Ascophyllum nodosum, temperature, illumination, tidal cycle, polar day, Barents Sea