Исследование микробных сообществ в подповерхностных слоях донных отложений шельфовой и глубоководной зоны Чёрного моря с использованием OxfordNanoporeсеквенирования

Abstract

An analysis was conducted on the bacterial communities present in the subsurface bottom sediment layers from the deep basin and shelf zone of the Black Sea. The study aimed to demonstrate that these sediments, formed under different bottom water conditions ‒ reduced environment characterized by hydrogen sulfide at a depth of 2,030 meters and oxidized environment with oxygenated seawater at a depth of 49 meters ‒ serve as habitats for distinct microbial communities. DNA was extracted from various sediment layers, followed by PCR amplification and sequencing using Oxford Nanopore technology. The analysis identified a total of twenty-eight classified bacterial phylotypes: twenty-seven were found in both zones, while one was specific to the deep sediment of the hydrogen sulfide zone. In total, 391 representatives were identified across 219 families. Among these, fifty-nine families were found to be specific to the shelf sediment of the oxygen zone, while 50 families were exclusive to the deep sediment of the hydrogen sulfide zone. Additionally, 110 families were identified as overlapping between both zones of the Black Sea. These findings indicate that the two zones of the Black Sea indeed harbor unique microbial communities, with greater diversity observed in the shelf zone. The results also highlight the potential for future research to explore the roles of these communities in biogeochemical cycles.

Keywords: The Black Sea, Core of bottom sediment, DNA concentration, sequencing by Oxford Nanopore method,prokaryote microbiome, subsurface bottom sediment, shelf oxygen-containing zone, hydrogen sulfide deep-sea zone