Биодеградация нонифенола бактерией Raoultella planticola F8, выделенной из донных осадков Финского залива Балтийского моря
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Аннотация
Нонилфенол (НФ) — ксенобиотик антропогенного происхождения — является широко распространённым во всём мире загрязнителем окружающей среды. Попадание НФ в объекты окружающей среды вызывает серьёзную озабоченность вследствие его токсичности для водных организмов, животных и человека. Кроме того, НФ известен как эндокринный деструктор. Цель данной статьи — выделение из донных отложений, отобранных в южной части Финского залива (Балтийское море), и идентификация высокоэффективного штамма бактерий, способного деструктировать НФ, а также изучение его способности к деградации НФ при различных уровнях температуры, pH, концентраций растворённого кислорода и исходных концентраций НФ. Идентификацию выделенного штамма F8 проводили по фенотипическим признакам с использованием стандартных методов, а также методом секвенирования по Сэнгеру фрагмента последовательности гена 16S рРНК (rrs). Содержание НФ определяли методом высокоэффективной жидкостной хроматографии. Новая НФ-деструктирующая бактерия Raoultella planticola F8 выделена из донных отложений, отобранных в Финском заливе. Изолят R. planticola F8 депонирован в Ведомственной коллекции микроорганизмов сельскохозяйственного назначения ВНИИСХМ под регистрационным номером RCAM 05450. Последовательность гена rrs изолята R. planticola F8 депонирована в базе данных GenBank под номером OL831016. Этот штамм высокоэффективен для деградации НФ в аэробных условиях при различных концентрациях НФ (до 900 мг·л−1), в диапазоне температур от +5 до +35 °C, начальных значений pH от 5 до 9 и концентраций растворённого кислорода от 0,8 до 2,46 мг·л−1. Данное исследование — первое, демонстрирующее способность R. planticola транформировать НФ. Результаты этой работы предоставляют полезную информацию для применения R. planticola F8 в процессах биоремедиации.
Авторы
Библиографические ссылки
Abatenh E., Gizaw B., Tsegaye Z., Wassie M. The role of microorganisms in bioremediation – a review. Open Journal of Environmental Microbiology, 2017, vol. 2, iss. 1, pp. 038–046. https://doi.org/10.17352/ojeb.000007
Alneyadi A. H., Shah I., AbuQamar S. F., Ashraf S. S. Differential degradation and detoxification of an aromatic pollutant by two different peroxidases. Biomolecules, 2017, vol. 7, iss. 1, art. no. 31 (18 p.). https://doi.org/10.3390/biom7010031
Bai N., Abuduaini R., Wang S., Zhang M., Zhu X., Zhao Y. Nonylphenol biodegradation characterizations and bacterial composition analysis of an effective consortium NP-M2. Environmental Pollution, 2017, vol. 220, pt A, pp. 95–104. https://doi.org/10.1016/j.envpol.2016.09.027
Baptista M. S., Stoichev T., Basto M. C. P., Vasconcelos V. M., Vasconcelos M. T. S. D. Fate and effects of octylphenol in a Microcystis aeruginosa culture medium. Aquatic Toxicology, 2009, vol. 92, iss. 2, pp. 59–64. https://doi.org/10.1016/j.aquatox.2008.12.005
Barber L. B., Loyo-Rosales J. E., Rice C. P., Minarik T. A., Oskouie A. K. Endocrine disrupting alkylphenolic chemicals and other contaminants in wastewater treatment plant effluents, urban streams, and fish in the Great Lakes and Upper Mississippi River Regions. Science of the Total Environment, 2015, vol. 517, pp. 195–206. https://doi.org/10.1016/j.scitotenv.2015.02.035
Bergey’s Manual of Determinative Bacteriology. 9th edition / D. H. Bergey, J. G. Holt (Eds). Baltimore : Williams & Wilkins, 1994, 787 p.
Bhandari G., Bagheri A. R., Bhatt P., Bilal M. Occurrence, potential ecological risks, and degradation of endocrine disrupter, nonylphenol, from the aqueous environment. Chemosphere, 2021, vol. 275, art. no. 130013 (16 p.). https://doi.org/10.1016/j.chemosphere.2021.130013
Bhatt P., Huang Y., Zhan H., Chen S. Insight into microbial applications for the biodegradation of pyrethroid insecticides. Frontiers in Microbiology, 2019, vol. 10, art. no. 1778 (18 p.). https://doi.org/10.3389/fmicb.2019.01778
Cao B., Nagarajan K., Loh K.-C. Biodegradation of aromatic compounds: Current status and opportunities for biomolecular approaches. Applied Microbiology and Biotechnology, 2009, vol. 85, iss. 2, pp. 207–228. https://doi.org/10.1007/s00253-009-2192-4
Corvini P. F. X., Schäffer A., Schlosser D. Microbial degradation of nonylphenol and other alkylphenols–our evolving view. Applied Microbiology and Biotechnology, 2006, vol. 72, iss. 2, pp. 223–243. https://doi.org/10.1007/s00253-006-0476-5
De Weert J., de la Cal A., van den Berg H., Murk A., Langenhoff A., Rijnaarts H., Grotenhuis T. Bioavailability and biodegradation of nonylphenol in sediment determined with chemical and bioanalysis. Environmental Toxicology and Chemistry, 2008, vol. 27, iss. 4, pp. 778–785. https://doi.org/10.1897/07-367.1
Drancourt M., Bollet C., Carta A., Rousselier P. Phylogenetic analyses of Klebsiella species delineate Klebsiella and Raoultella gen. nov., with description of Raoultella ornithinolytica comb. nov., Raoultella terrigena comb. nov. and Raoultella planticola comb. nov. International Journal of Systematic and Evolutionary Microbiology, 2001, vol. 51, iss. 3, pp. 925–932. https://doi.org/10.1099/00207713-51-3-925
Feng Y., Wang A., Fu W., Song D. F. Growth performance, antioxidant response, biodegradation and transcriptome analysis of Chlorella pyrenoidosa after nonylphenol exposure. Science of the Total Environment, 2022, vol. 806, pt 1, art. no. 150507 (9 p.). https://doi.org/10.1016/j.scitotenv.2021.150507
Gabriel F. L., Giger W., Guenther K., Kohler H. P. Differential degradation of nonylphenol isomers by Sphingomonas xenophaga Bayram. Applied and Environmental Microbiology, 2005, vol. 71, no. 3, pp. 1123–1129. https://doi.org/10.1128/AEM.71.3.1123-1129.2005
Ibrahim M., Makky E. A., Azmi N. S., Ismail J. Optimization parameters for Mycobacteria confluentis biodegradation of PAHs. MATEC Web of Conferences, 2018, vol. 150, art. no. 06035 (5 p.). https://doi.org/10.1051/matecconf/201815006035
Karigar C. S., Rao S. S. Role of microbial enzymes in the bioremediation of pollutants: A review. Enzyme Research, 2011, vol. 2011, art. no. 805187 (11 p.). https://doi.org/10.4061/2011/805187
Khalid M., Abdollahi M. Environmental distribution of personal care products and their effects on human health. Iranian Journal of Pharmaceutical Research, 2021, vol. 20, iss. 1, pp. 216–253. https://doi.org/10.22037/ijpr.2021.114891.15088
Khan S. A., Hamayun M., Khan A. L., Ahmad B., Ahmed S., Lee I.-J. Influence of pH, temperature and glucose on biodegradation of 4-aminophenol by a novel bacterial strain, Pseudomonas sp. ST-4. African Journal of Biotechnology, 2009, vol. 8, no. 16, pp. 3827–3831.
Kimura Z., Chung K. M., Itoh H., Hiraishi A., Okabe S. Raoultella electrica sp. nov., isolated from anodic biofilms of a glucose-fed microbial fuel cell. International Journal of Systematic and Evolutionary Microbiology, 2014, vol. 64, iss. pt_4, pp. 1384–1388. https://doi.org/10.1099/ijs.0.058826-0
Krige N. R., Padgtt P. J. Phenotypic and physiological characterization methods. In: Taxonomy of Prokaryotes / F. Rainey, A. Oren (Eds). London ; San Diego ; Waltham ; Amsterdam : Academic Press, 2011, pp. 15–60. (Methods in Microbiology ; vol. 38). https://doi.org/10.1016/B978-0-12-387730-7.00003-6
Kuzikova I., Rybalchenko O., Kurashov E., Krylova Y., Safronova V., Medvedeva N. Defense responses of the marine-derived fungus Aspergillus tubingensis to alkylphenols stress. Water, Air, & Soil Pollution. An International Journal of Environmental Pollution, 2020, vol. 231, iss. 6, art. no. 271 (18 p.). https://doi.org/10.1007/s11270-020-04639-2
Lakshmi M. V. V. C., Sridevi V. Effect of pH and inoculum size on phenol degradation by Pseudomonas aeruginosa (NCIM 2074). International Journal of Chemical Sciences, 2009, vol. 7, iss. 4, pp. 2246–2252.
Liu Y., Chang H., Li Z., Feng Y., Cheng D., Xue J. Biodegradation of gentamicin by bacterial consortia AMQD4 in synthetic medium and raw gentamicin sewage. Scientific Reports, 2017, vol. 7, art. no. 11004 (11 p.). https://doi.org/10.1038/s41598-017-11529-x
Ma J., Chen F., Tang Y., Wang X. Research on degradation characteristics of nonylphenol in water by highly effective complex microorganisms. E3S Web of Conferences, 2018, vol. 53, art. no. 04016 (7 p.). https://doi.org/10.1051/e3sconf/20185304016
Mao Z., Zheng X.-F., Zhang Y.-Q., Tao X.-X., Li Y., Wang W. Occurrence and biodegradation of nonylphenol in the environment. International Journal of Molecular Sciences, 2012, vol. 13, iss. 1, pp. 491–505. https://doi.org/10.3390/ijms13010491
Palyzová A., Zahradník J., Marešová H., Řezanka T. Characterization of the catabolic pathway of diclofenac in Raoultella sp. KDF8. International Biodeterioration & Biodegradation, 2019, vol. 137, pp. 88–94. https://doi.org/10.1016/j.ibiod.2018.11.013
Ping L., Guo Q., Chen X., Yuan X., Zhang C., Zhao H. Biodegradation of pyrene and benzo[a]pyrene in the liquid matrix and soil by a newly identified Raoultella planticola strain. 3 Biotech, 2017, vol. 7, iss. 1, art. no. 56 (10 p.). https://doi.org/10.1007/s13205-017-0704-y
Rajendran R. K., Huang S.-L., Lin C.-C., Kirschner R. Biodegradation of the endocrine disrupter 4-tert-octylphenol by the yeast strain Candida rugopelliculosa RRKY5 via phenolic ring hydroxylation and alkyl chain oxidation pathways. Bioresource Technology, 2017, vol. 226, pp. 55–64. https://doi.org/10.1016/j.biortech.2016.11.129
Reddy M. V., Yajima Y., Choi D. B., Chang Y.-C. Biodegradation of toxic organic compounds using a newly isolated Bacillus sp. CYR2. Biotechnology and Bioprocess Engineering, 2017, vol. 22, iss. 3, pp. 339–346. https://doi.org/10.1007/s12257-017-0117-0
Soares A., Guieysse B., Jefferson B., Cartmell E., Lester J. N. Nonylphenol in the environment: A critical review on occurrence, fate, toxicity and treatment in wastewaters. Environment International, 2008, vol. 34, iss. 7, pp. 1033–1049. https://doi.org/10.1016/j.envint.2008.01.004
Solé M., López de Alda M. J., Castillo M., Porte C., Ladegaard-Pedersen K., Barceló D. Estrogenicity determination in sewage treatment plants and surface waters from the Catalonian area (NE Spain). Environmental Science & Technology, 2000, vol. 34, iss. 24, pp. 5076–5083. https://doi.org/10.1021/es991335n
Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. MEGA5: Molecular Evolutionary Genetics Analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution, 2011, vol. 28, iss. 10, pp. 2731–2739. https://doi.org/10.1093/molbev/msr121
Tuan N. N., Hsieh H.-C., Lin Y.-W., Huang S.-L. Analysis of bacterial degradation pathways for long-chain alkylphenols involving phenol hydroxylase, alkylphenol monooxygenase and catechol dioxygenase genes. Bioresource Technology, 2011, vol. 102, iss. 5, pp. 4232–4240. https://doi.org/10.1016/j.biortech.2010.12.067
Uĝuz C., İşcan M., Togan İ. Alkylphenols in the environment and their adverse effects on living organisms. Kocatepe Veterinary Journal, 2009, vol. 2, iss. 1, pp. 49–58.
Vallini G., Frassinetti S., D’Andrea F., Catelani G., Agnolucci M. Biodegradation of 4-(1-nonyl)phenol by axenic cultures of the yeast Candida aquaetextoris: Identification of microbial breakdown products and proposal of a possible metabolic pathway. International Biodeterioration & Biodegradation, 2001, vol. 47, iss. 3, pp. 133–140. https://doi.org/10.1016/S0964-8305(01)00040-3
Wang Z., Yang Y., Dai Y., Xie S. Anaerobic biodegradation of nonylphenol in river sediment under nitrate- or sulfate-reducing conditions and associated bacterial community. Journal of Hazardous Materials, 2015a, vol. 286, pp. 306–314. https://doi.org/10.1016/j.jhazmat.2014.12.057
Wang Z., Yang Y., He T., Xie S. Change of microbial community structure and functional gene abundance in nonylphenol-degrading sediment. Applied Microbiology and Biotechnology, 2015b, vol. 99, iss. 7, pp. 3259–3268. https://doi.org/10.1007/s00253-014-6222-5
Watanabe W., Hori Y., Nishimura S., Takagi A., Kikuchi M., Sawai J. Bacterial degradation and reduction in the estrogen activity of 4-nonylphenol. Biocontrol Science, 2012, vol. 17, iss. 3, pp. 143–147. https://doi.org/10.4265/bio.17.143
Water Quality – Determination of Dissolved Oxygen – Iodometric Method (ISO Standard No. 5813:1983). ISO. International Organization for Standardization, 1983. https://www.iso.org/standard/11959.html
Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. 16S ribosomal DNA amplification for phylogenetic study. Journal of Bacteriology, 1991, vol. 173, no. 2, pp. 697–703. https://doi.org/10.1128/jb.173.2.697-703.1991
Xie Y., Pan Y., Bai B., Xu Z., Ding L., Li Q., Xu Y., Zhu T. Degradation performance and optimal parameters of two bacteria in degrading nonylphenol. Journal of Computational and Theoretical Nanoscience, 2015, vol. 12, no. 9, pp. 2657–2663. https://doi.org/10.1166/jctn.2015.4159
Yang Z., Shi Y., Zhang Y., Cheng Q., Li X., Zhao C., Zhang D. Different pathways for 4-n-nonylphenol biodegradation by two Aspergillus strains derived from estuary sediment: Evidence from metabolites determination and key-gene identification. Journal of Hazardous Materials, 2018, vol. 359, pp. 203–212. https://doi.org/10.1016/j.jhazmat.2018.07.058
Zaytseva T. B., Medvedeva N. G. Sorption and biodegradation of octyl- and nonylphenols by the cyanobacterium Planktothrix agardhii (Gomont) Anagn. & Komárek. Inland Water Biology, 2019, vol. 12, iss. 3, pp. 337–345. https://doi.org/10.1134/s1995082919030192
