Биодеградация нонифенола бактерией Raoultella planticola F8, выделенной из донных осадков Финского залива Балтийского моря
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Аннотация
Нонилфенол (НФ) — ксенобиотик антропогенного происхождения — является широко распространённым во всём мире загрязнителем окружающей среды. Попадание НФ в объекты окружающей среды вызывает серьёзную озабоченность вследствие его токсичности для водных организмов, животных и человека. Кроме того, НФ известен как эндокринный деструктор. Цель данной статьи — выделение из донных отложений, отобранных в южной части Финского залива (Балтийское море), и идентификация высокоэффективного штамма бактерий, способного деструктировать НФ, а также изучение его способности к деградации НФ при различных уровнях температуры, pH, концентраций растворённого кислорода и исходных концентраций НФ. Идентификацию выделенного штамма F8 проводили по фенотипическим признакам с использованием стандартных методов, а также методом секвенирования по Сэнгеру фрагмента последовательности гена 16S рРНК (rrs). Содержание НФ определяли методом высокоэффективной жидкостной хроматографии. Новая НФ-деструктирующая бактерия Raoultella planticola F8 выделена из донных отложений, отобранных в Финском заливе. Изолят R. planticola F8 депонирован в Ведомственной коллекции микроорганизмов сельскохозяйственного назначения ВНИИСХМ под регистрационным номером RCAM 05450. Последовательность гена rrs изолята R. planticola F8 депонирована в базе данных GenBank под номером OL831016. Этот штамм высокоэффективен для деградации НФ в аэробных условиях при различных концентрациях НФ (до 900 мг·л−1), в диапазоне температур от +5 до +35 °C, начальных значений pH от 5 до 9 и концентраций растворённого кислорода от 0,8 до 2,46 мг·л−1. Данное исследование — первое, демонстрирующее способность R. planticola транформировать НФ. Результаты этой работы предоставляют полезную информацию для применения R. planticola F8 в процессах биоремедиации.
Авторы
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